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Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil

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Abstract

We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex (Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito pool was collected. Flavivirus RNA was detected by real-time RT-PCR of homogenized mosquitoes and from the corresponding C6/36 culture supernatant. Based on full-genome sequencing, the virus isolate was genetically distinct from but most closely related to Barkedji virus (BJV), a newly described flavivirus from Senegal. Phylogenetic analysis demonstrated that NHUV grouped with mosquito-borne flaviviruses forming a clade with BJV. This clade may be genetically intermediate between the Culex-borne flaviviruses amplified by birds and the insect-only flaviviruses.

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Acknowledgements

Special thanks to Zilca Campos from Embrapa Pantanal for her important contribution to this work. We thank Kristy Burkalther, Janae Stovall, Karen Burroughs and Robert Lanciotti from CDC, who provided laboratory assistance. We thank Thierry Tomich, Marcia Furlan, Marcos Tadeu and all staff from Embrapa Pantanal. Special thanks to Hélcio Reinaldo Gil-Santana from Diptera Laboratory of FIOCRUZ, and Luiz Pellegrin of Geoprocessing Laboratory of Embrapa Pantanal. Financial support: ASM, CNPq, CAPES, Fulbright, CDC, FIOCRUZ, FAPERJ.

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratório de Flavivírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Ministério da Saúde, Rio de Janeiro, RJ, Brazil

    Alex Pauvolid-Corrêa, Dinair Couto-Lima & Rita Nogueira

  2. Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, USA

    Alex Pauvolid-Corrêa, Joan Kenney, Aaron Brault & Nicholas Komar

  3. Systems Biology Department, Sandia National Laboratories, Livermore, CA, USA

    Owen Solberg & Stanley Langevin

  4. Laboratório de Referência Nacional para Vetores das Riquetsioses, Fundação Oswaldo Cruz, Ministério da Saúde, Rio de Janeiro, RJ, Brazil

    Nicolau Serra-Freire

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  1. Alex Pauvolid-Corrêa
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  2. Owen Solberg
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  3. Dinair Couto-Lima
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Correspondence to Alex Pauvolid-Corrêa.

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Alex Pauvolid-Correˆa was a fulbright doctorate student at CDC.

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Pauvolid-Corrêa, A., Solberg, O., Couto-Lima, D. et al. Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil. Arch Virol 160, 21–27 (2015). https://doi.org/10.1007/s00705-014-2219-8

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