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Complete genome sequence of a novel ourmia-like mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea

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Abstract

Here, we describe the full-length genome sequence of a novel ourmia-like mycovirus, tentatively named “Botryosphaeria dothidea ourmia-like virus 1” (BdOLV1), isolated from the phytopathogenic fungus Botryosphaeria dothidea strain 8A, associated with apple ring rot in Shanxi province, China. The complete BdOLV1 genome is comprised of a 2797-nucleotide positive-sense (+) single-stranded RNA (ssRNA) with a single open reading frame (ORF). The ORF putatively encodes a 642-amino-acid polypeptide with conserved RNA-dependent RNA polymerase (RdRp) motifs related to those of viruses of the family Botourmiaviridae. Phylogenetic analysis based on RdRp amino acid sequences showed that BdOLV1 is grouped with unclassified oomycete-infecting viruses closely related to members of the genus Botoulivirus in the family Botourmiaviridae. This is the first report of a novel (+)ssRNA virus in B. dothidea related to members of the genus Botoulivirus in the family Botourmiaviridae.

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Acknowledgements

We greatly thank Dr. Guangyu Sun for kindly providing research materials. This work was supported in part by the National Natural Science Foundation of China (30970163) to LY. Sun and (31970159), to IB. Andika.

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Author notes

  1. Ziqian Lian and Subha Das contributed equally to the work.

Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China

    Ziqian Lian, Subha Das, Jingxian Luo & Liying Sun

  2. College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, 266109, China

    Ida Bagus Andika

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  1. Ziqian Lian
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  2. Subha Das
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  3. Jingxian Luo
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  4. Ida Bagus Andika
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  5. Liying Sun
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Correspondence to Liying Sun.

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Lian, Z., Das, S., Luo, J. et al. Complete genome sequence of a novel ourmia-like mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea. Arch Virol 166, 3461–3465 (2021). https://doi.org/10.1007/s00705-021-05221-9

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  • DOI: https://doi.org/10.1007/s00705-021-05221-9

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