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Hydrogen sulfide is involved in maintaining ion homeostasis via regulating plasma membrane Na+/H+ antiporter system in the hydrogen peroxide-dependent manner in salt-stress Arabidopsis thaliana root

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Abstract

Hydrogen sulfide (H2S) and hydrogen peroxide (H2O2) function as the signaling molecules in plants responding to salt stresses. The present study presents a signaling network involving H2S and H2O2 in salt resistance pathway of the Arabidopsis root. Arabidopsis roots were sensitive to 100 mM NaCl treatment, which displayed a great increase in electrolyte leakage (EL) and Na+/K+ ratio under salt stress. The treatment of H2S donors sodium hydrosulfide (NaHS) enhanced the salt tolerance by maintaining a lower Na+/K+ ratio. In addition, the inhibition of root growth under salt stress was removed by H2S. Further studies indicated that H2O2 was involved in H2S-induced salt tolerance pathway. H2S induced the production of the endogenous H2O2 via regulating the activities of glucose-6-phosphate dehydrogenase (G6PDH) and plasma membrane (PM) NADPH oxidase, with the treatment with dimethylthiourea (DMTU, an ROS scavenger), diphenylene iodonium (DPI, a PM NADPH oxidase inhibitor), or glycerol (G6PDH inhibitor) removing the effect of H2S. Treatment with amiloride (an inhibitor of PM Na+/H+ antiporter) and vanadate (an inhibitor of PM H+-ATPase) also inhibited the activity of H2S on Na+/K+ ratio. Through an analysis of quantitative real-time polymerase chain reaction and Western blot, we found that H2S promoted the genes expression and the phosphorylation level of PM H+-ATPase and Na+/H+ antiporter protein level. However, when the endogenous H2O2 level was inhibited by DPI or DMTU, the effect of H2S on the PM Na+/H+ antiporter system was removed. Taken together, H2S maintains ion homeostasis in the H2O2-dependent manner in salt-stress Arabidopsis root.

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Acknowledgments

Support for this work is from the Northwest A&F University doctor start-up Foundation (Z109021116).

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Authors and Affiliations

  1. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China

    Jisheng Li, Honglei Jia, Qianhua Cao & Zichao Wen

  2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Jue Wang

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  1. Jisheng Li
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  2. Honglei Jia
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Correspondence to Jisheng Li.

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Handling Editor: Peter Nick

Jisheng Li and Honglei Jia these two authors contributed equally to this work.

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Li, J., Jia, H., Wang, J. et al. Hydrogen sulfide is involved in maintaining ion homeostasis via regulating plasma membrane Na+/H+ antiporter system in the hydrogen peroxide-dependent manner in salt-stress Arabidopsis thaliana root. Protoplasma 251, 899–912 (2014). https://doi.org/10.1007/s00709-013-0592-x

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