Abstract
Amino acids are one of the main metabolites in the body, and provide energy for the body and brain. The purpose of this study is to provide a profile of amino acid changes in the serum of patients with Moyamoya disease (MMD) and identify potential disease biomarkers. In this paper, we quantitatively determined the serum amino acid metabolic profiles of 43 MMD patients and 42 healthy controls (HCs). T test, multivariate statistical analysis, and receiver operating characteristic (ROC) curve analysis were used to identify candidate markers. Thirty-nine amino acids were quantified, and 12 amino acid levels differed significantly between the MMD patients and HCs. Moreover, based on ROC curve analysis, four amino acid (l-methionine, l-glutamic acid, β-alanine and o-phosphoserine) biomarkers showed high sensitivity and specificity (AUC > 0.90), and showed the best sensitivity and specificity in MetaboAnalyst 5.0 using binary logistic regression analysis. We have provided serum amino acid metabolic profiles of MMD patients, and identified four potential biomarkers which may both provide clinicians with an objective diagnostic method for early detection of MMD and further our understanding of MMD pathogenesis.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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We appreciate all the participants and the staffs of the Department of Clinical Translational Medicine, Jining Life Science Center for their invaluable support and assistance.
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Our work was supported by Ph.D. Research Foundation of the Affiliated Hospital of Jining Medical University (no. 2021-BS-014), the Taishan Scholar Project of Shandong Province (no. tsqn201812159), the Natural Science Foundation of China (no. 81602846; no. 81901954), and the Key Research and Development Program of Jining Science and Technology (no. 2019SMNS012).
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PJ and CC conceptualized the research and designed the study. CW, SZ, and SH collected and collated the data. XL, FJ, and PJ analyzed and interpreted the data. XL, FJ, and SZ performed statistical analyses. XL drafted the manuscript. PJ and CC critically revised the manuscript, and all authors read and approved the final manuscript. PJ is the guarantor of this work and as such had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.
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Our work was based on the Declaration of Helsinki and was agreed upon by all participants, with written informed consent. In addition, this study was approved by the Ethics Committee of The Affiliated Hospital of Jining Medical University (Jining, China) (Approval No. 2017C036).
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Liu, X., Jin, F., Wang, C. et al. Targeted metabolomics analysis of serum amino acid profiles in patients with Moyamoya disease. Amino Acids 54, 137–146 (2022). https://doi.org/10.1007/s00726-021-03100-w
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DOI: https://doi.org/10.1007/s00726-021-03100-w