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Halomonas sediminis sp. nov., a new halophilic bacterium isolated from salt-lake sediment in China

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Abstract

A novel Gram-negative, slightly halophilic, catalase- and oxidase-positive, obligately aerobic bacterium, strain YIM-C248T, was isolated from a sediment sample collected from a salt-lake in the Qaidam Basin in Qinghai, north-west China. Cells were non-sporulating short rods, occurring singly or as doublets, motile with peritrichous flagella. Growth occurred with 1–15% (w/v) NaCl [optimum 2–4% (w/v) NaCl], at pH 6.0–10.0 (optimum pH 7.5) and at 4–35°C (optimum 25–30°C). The major cellular fatty acids were C18:1 ω7c, C12:0 3-OH, cyclo C19:0 ω8c, C16:0 and C16:1. The predominant respiratory quinone was Q-9 and the genomic DNA G + C content was 58.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM-C248T should be assigned to the genus Halomonas. The sequence similarities between the isolate and the type strains of members of the genus Halomonas were in the range of 92.5–97.5%. The combination of phylogenetic analysis, DNA–DNA hybridization data, phenotypic characteristics and chemotaxonomic differences supported the view that strain YIM-C248T represents a new species of the genus Halomonas, for which the name Halomonas sediminis sp. nov. is proposed, with YIM-C248T (=CCTCC AA 207031 = KCTC 22167) as the type strain.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NSFC) (30860013, 30660004, 30760006), the Ministry of Science and Technology of China (863 Program, no. 2007AA021306), Jishou University (jsdxkyzz200801, 07JDPHE148, 07JDPHE150, 07JDPHE151, 07JDPHE153), and the Yunnan Provincial Sciences and Technology Department (2005PY01-1, 2006C0006M). We are grateful to Mr. Huai-Dong Xiao and Mr. Ke Huang for their excellent technical assistance.

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Authors and Affiliations

  1. Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, 650091, Kunming, Yunnan, People’s Republic of China

    Heng-Yu Huang, Yi-Guang Chen, Yong-Xia Wang, Ji-Hui Liu, Shu-Kun Tang, Qian Peng, Meng-Liang Wen, Hong Yu & Xiao-Long Cui

  2. College of Bio-resources and Environmental Science, Jishou University, 416000, Jishou, Hunan, People’s Republic of China

    Heng-Yu Huang & Yi-Guang Chen

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  1. Heng-Yu Huang
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  2. Yi-Guang Chen
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  3. Yong-Xia Wang
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  4. Ji-Hui Liu
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  5. Shu-Kun Tang
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  6. Qian Peng
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  7. Meng-Liang Wen
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  8. Hong Yu
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  9. Xiao-Long Cui
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Corresponding authors

Correspondence to Hong Yu or Xiao-Long Cui.

Additional information

Communicated by H. Santos.

The GenBank/EMBL/DBBJ accession number for the 16S rRNA gene sequence of strain YIM-C248T is EU135707.

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Huang, HY., Chen, YG., Wang, YX. et al. Halomonas sediminis sp. nov., a new halophilic bacterium isolated from salt-lake sediment in China. Extremophiles 12, 829–835 (2008). https://doi.org/10.1007/s00792-008-0189-z

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  • DOI: https://doi.org/10.1007/s00792-008-0189-z

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