Abstract
Physical, chemical, and biological properties of wood depend largely on the properties of cellulose, noncellulosic polysaccharides, and lignin, and their assembly mode in the cell wall. Information on the assembly mode in the main part of the ginkgo tracheid wall (middle layer of secondary wall, S2) was drawn from the combined results obtained by physical and chemical analyses of the mechanically isolated S2 and by observation under scanning electron microscopy. A schematic model was tentatively proposed as a basic assembly mode of cell wall polymers in the softwood tracheid as follows: a bundle of cellulose microfibrils (CMFs) consisting of about 430 cellulose chains is surrounded by bead-like tubular hemicellulose-lignin modules (HLM), which keep the CMF bundles equidistant from each other. The length of one tubular module along the CMF bundle is about 16 ± 2 nm, and the thickness at its side is about 3–4 nm. In S2, hemicelluloses are distributed in a longitudinal direction along the CMF bundle and in tangential and radial directions perpendicular to the CMF bundle so that they are aligned in the lamellae of tangential and radial directions with regard to the cell wall. One HLM contains about 7000 C6-C3 units of lignin, and 4000 hexose and 2000 pentose units of hemicellulose.
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Terashima N, Fukushima K, He LF, Takabe K (1993) Comprehensive model of the lignified plant cell wall. In: Jung HG, Buxton DR, Hatfield RD, Ralph J (eds) Forage cell wall structure and digestibility. American Society of Agronomy, Madison, USA, pp 247–270
Timell TE (1986) Origin and evolution of compression wood. In: Compression wood in gymnosperms. Springer, Berlin Heidelberg New York, pp 597–621
Terashima N (2007) Non-destructive approaches to identify the ultrastructure of lignified ginkgo cell walls. Int J Plant Develop Biol 1:170–177
Terashima N, Akiyama T, Ralph S, Evtuguin D, Pascoal Neto C, Parkås J, Paulsson M, Westermark U, Ralph J (2009) 2D-NMR (HSQC) difference spectra between specifically 13C-enriched and unenriched protolignin of Ginkgo biloba obtained in the solution state of whole cell wall material. Holzforschung 63:379–384
Terashima N, Yoshida M (2006) Observation of formation process of macromolecular lignin in the cell wall by electron microscope IV. Formation of hemicellulose-lignin module in black pine tracheid. Proceedings of the Annual Meeting of the Japan Wood Research Society Akita, Japan, PA005
Terashima N, Awano T, Takabe T, Yoshida M (2004) Formation of macromolecular lignin in ginkgo xylem cell walls as observed by field emission scanning electron microscopy. Comptes Rendus Biologies 327:903–910
Terashima N, Yoshida M (2005) Ultrastructural assembly of polysaccharides and lignin in lignifying plant cell walls. Proceedings of 13th International Symposium on Wood, Fiber, and Pulping Chemistry, Auckland, New Zealand, vol 2, pp 423–426
Browning BL (1967) Preparation of holocellulose by chlorite methods (Wise method) and determination of alpha-cellulose content. In: Methods of wood chemistry, vol 2. Interscience, New York, p 395, 418
Dence CW (1992) Determination of lignin in wood and pulp by the acetyl bromide method. In: Lin SY, Dence CW (eds) Methods in lignin chemistry, Springer, Berlin Heidelberg New York, pp 44–48
Yamamoto H, Okuyama T, Yoshida M (1993) Method of determining the mean microfibril angle of wood over a wide range by the improved Cave’s method. Mokuzai Gakkaishi 39:375–381
Hafrén J, Fujino T, Itoh T (1999) Changes in cell wall architecture of differentiating tracheids of Pinus thunbergiii during lignification. Plant Cell Physiol 40:532–541
Fahlén J, Salmén L (2003) Cross-sectional structure of the secondary wall of wood fibers as affected by processing. J Mater Sci 38:119–126
Nishiyama Y, Langan P, Chanzy H (2002) Crystal structure and hydrogen-bonding system in cellulose Iβ from synchrotron X-ray and neutron fiber diffraction. J Am Chem Soc 124:9074–9082
Kataoka Y, Kondo T (1996) Changing cellulose crystalline structure in forming wood cell walls. Macromolecules 29:6356–6358
Herth W (1983) Arrays of plasma-membrane “grosettes” involved in cellulose microfibril formation of Spyrogyra. Planta 159:347–356
Sugiyama J, Harada H, Fujiyoshi Y, Uyeda N (1985) Lattice images from ultrathin sections of cellulose microfibrils in the cell wall of Valonia macrophysa Kütz. Planta 166:161–168
Helbert W, Nishiyama Y, Okano T, Sugiyama J (1998) Molecular imaging of Halocynthia papillosa cellulose. J Struct Biol 124:42–50
Xu P, Donaldson LA, Gergely ZR, Staehelin A (2007) Dual axis electron tomography: a new approach for investigating the special organization of wood cellulose microfibrils. Wood Sci Technol 41:101–116
Baker AA, Helbert W, Sugiyama J, Miles MJ (2000) New insight into cellulose structure by atomic force microscope shows the Iα crystal phase at near-atomic resolution. Biophys J 79:1139–1145
Timell TE (1960) Studies on Ginkgo biloba L. 1. General characteristics and chemical composition. Sven Papperstidn 63:652–657
Mian J, Timell TE (1960) Studies on Ginkgo biloba L. 2. The constitution of an arabino-4-O-methyl-glucurono-xylan from the wood. Sven Papperstidn 63:769–774
Timell TE (1961) Isolation of galactoglucomannans from the wood of gymnosperms. TAPPI 44:88–96
Yoshida M, Hosoo Y, Okuyama T (2000) Periodicity as a factor in the generation of isotropic compressive growth stress between microfibrils in cell wall formation during a twenty-four hour period. Holzforschung 54:469–473
Hosoo Y, Imai T, Yoshida M (2006) Diurnal differences in the supply of glucomannanns and xylans in inner-most surface of cell walls at various developmental stages from cambium to mature xylem in Cryptomeria japonica. Protoplasma 229:11–19
Tokoh C, Takabe K, Sugiyama J, Fujita M (2002) Cellulose synthesized by Acetobacter xylinum in the presence of plant cell wall polysaccharides. Cellulose 9:65–74
Tokoh C, Takabe K, Sugiyama J, Fujita M (2002) CP/MAS 13C NMR and electron diffraction study of bacterial cellulose structure affected by cell wall polysaccharides. Cellulose 9:351–360
Awano T, Takabe Y, Fujita M (2002) Xylan deposition on secondary wall of Fagus crenata fiber. Protoplasma 219:106–115
Ralph J, Grabber JH, Hatfield RD (1995) Lignin-ferulate crosslink in grasses: active incorporation of ferulate polysaccharide esters into ryegrass lignins. Carbohydr Res 275:167–178
Ralph J, Hatfield RD, Grabber JH, Jung H-JG, Quideau S, Helm RF (1998) Cell wall cross-linking in grasses by ferulates and diferulates. In: Lewis NG, Sarkanen S (eds) ACS Symposium Series 697, Lignin and lignan biosynthesis. American Chemical Society, Washington DC, pp 209–236
Ramiah MV, Goring DAI (1965) The thermal expansion of cellulose, hemicellulose, and lignin. J Polym Sci Part C 11:27–48
Terashima N, Yoshida M (2006) Ultrastructure of lignified plant cell wall observed by field-emission scanning electron microscopy. Observations on periodate lignin prepared from Ginkgo biloba. Cellulose Chem Technol 40:727–733
Atalla RH, Agarwal UP (1986) Raman microprobe evidence for lignin orientation in the cell wall of native woody tissue. Science 227:636–638
Agarwal UP, Atalla RH (1986) In-situ Raman microprobe studies of plant cell walls: macromolecular organization and compositional variability in the secondary wall of Picea mariana (Mill.) B.S.P. Planta 169:325–332
Terashima N, Attala RH (1995) Formation and structure of plant cell wall - factors controlling lignin structure during its formation. Proceedings of the 8th International Symposium on Wood and Pulping Chemistry, Helsinki. Finland, vol 1, pp 69–76
Akiyama T, Ralph J (2008) Characteristics in 1H- and 13C-NMR chemical shifts of non-phenolic dibenzodioxocin model compounds as branch-points in lignin. Proceedings of 53rd Lignin Symposium, Tokyo, pp 84–87
Kukkola E, Koutaniemi S, Pollanen E, Gustafson M, Karuhnen P, Lundell TK, Saranpää P, Kilpäinen I, Teeri TH, Fagerstedt KV (2004) The dibenzodioxocin lignin substructure is abundant in the inner part of the secondary wall in Norway spruce and silver birch xylem. Planta 218:497–500
Kukkola E, Saranpää P, Fagerstedt K (2008) Juvenile and compressed wood cell walls layers differ in lignin structure in Norway spruce and Scots pine. IAWA J 29:47–54
Donaldson L (2007) Cellulose microfibril aggregates and their size variation with cell wall type. Wood Sci Technol 41:443–460
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Terashima, N., Kitano, K., Kojima, M. et al. Nanostructural assembly of cellulose, hemicellulose, and lignin in the middle layer of secondary wall of ginkgo tracheid. J Wood Sci 55, 409–416 (2009). https://doi.org/10.1007/s10086-009-1049-x
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DOI: https://doi.org/10.1007/s10086-009-1049-x