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The effect of Bacillus subtilis mouth rinsing in patients with periodontitis

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Abstract

Bacillus subtilis is an effective probiotic product for prevention of enteric infections both in humans and animals. We hypothesized that a mouth rinse containing Bacillus subtilis should adhere to and colonize part of the oral bacteria on periodontal tissue. The rinsing ability of Extraction 300E (containing Bacillus subtilis: E-300 ) was compared with that of a mouth wash liquid , Neosteline Green (benzethonium chloride; NG) that is commonly used in Japan. Compared with NG rinsing, E-300 rinsing resulted in a marked change in the BANA-score. The mean BANA values (score ± SD) over the course of the study from 0 to 30 days were 1.52 ± 0.51 (p ≤ 0.1) and 0.30 ± 0.47 (p ≤ 0.01) for E-300, and 1.56 ± 0.51 and 0.93 ± 0.68 for NG, respectively. Gingival Index also had improvement, while probing pocket depth and bleeding on probing showed small improvements. Mouth rinsing with E-300 significantly reduced periodontal pathogens compared with NG. These results suggest that Bacillus subtilis is an appropriate mouth rinse for patients with periodontitis.

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Authors and Affiliations

  1. Tsubura Dental Clinic, 9-6 Enomachi, Utsunomiya, Tochigi, 320-0802, Japan

    S. Tsubura

  2. Mizunuma Dental Clinic, 1490-2 Motegi, Motegimachi, Tochigi, 321-3531, Japan

    H. Mizunuma

  3. Ishikawa Dental Clinic, 1-8-4 Kanai, Nasukarasuyama, Tochigi, 321-0628, Japan

    S. Ishikawa

  4. Oyake Dental Clinic, 2815 Himuro, Utsunomiya, Tochigi, 321-3232, Japan

    I. Oyake

  5. Okabayashi Dental Clinic, 6-6 Hatsune, Nasukarasuyama, Tochigi, 321-0628, Japan

    M. Okabayashi

  6. Katoh Katsuma Dental Clinic, 1129-2 Ohta, Takanezawa, Shioya, Tochigi, 329-1217, Japan

    K. Katoh

  7. Shibata Dental Clinic, 271-15 Imaichi, Nikko, Tochigi, 321-1261, Japan

    M. Shibata

  8. AHC Co., Ltd, 343-1 Kosogimachi, Maebashi, Gunma, 371-0831, Japan

    T. Iizuka, T. Toda & T. Iizuka

Authors
  1. S. Tsubura
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  2. H. Mizunuma
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  3. S. Ishikawa
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  4. I. Oyake
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  5. M. Okabayashi
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  6. K. Katoh
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  7. M. Shibata
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  8. T. Iizuka
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  9. T. Toda
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  10. T. Iizuka
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Correspondence to S. Tsubura.

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Tsubura, S., Mizunuma, H., Ishikawa, S. et al. The effect of Bacillus subtilis mouth rinsing in patients with periodontitis. Eur J Clin Microbiol Infect Dis 28, 1353–1356 (2009). https://doi.org/10.1007/s10096-009-0790-9

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  • DOI: https://doi.org/10.1007/s10096-009-0790-9

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