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Photo-biomodulatory response of low-power laser irradiation on burn tissue repair in mice

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Abstract

The present work reports the photo-biomodulatory effect of red (632.8 nm) and near infrared (785 and 830 nm) lasers on burn injury in Swiss albino mice. Animals were induced with a 15-mm full thickness burn injury and irradiated with various fluences (1, 2, 3, 4, and 6 J/cm2) of each laser wavelength under study having a constant fluence rate (8.49 mW/cm2). The size of the injury following treatment was monitored by capturing the wound images at regular time intervals until complete healing. Morphometric assessment indicated that the group treated with 3-J/cm2 fluence of 830 nm had a profound effect on healing as compared to untreated controls and various fluences of other wavelengths under study. Histopathological assessment of wound repair on treatment with an optimum fluence (3 J/cm2) of 830 nm performed on days 2, 6, 12, and 18 post-wounding resulted in enhanced wound repair with migration of fibroblasts, deposition of collagen, and neovascularization as compared to untreated controls. The findings of the present study have clearly demonstrated that a single exposure of 3-J/cm2 fluence at 830-nm enhanced burn wound healing progression in mice, which is equivalent to 5 % povidone iodine treatment (reference standard), applied on a daily basis till complete healing.

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Acknowledgements

The authors would like to thank DAE-BRNS, Govt. of India for funding (Grant no. 2012/34/47). Authors also thank Prof. K. Satyamoorthy, Director, School of Life Sciences (SLS) for his support and Manipal University for providing necessary facilities at School of Life Sciences, Manipal University to conduct the study. We extend our gratitude to Dr. Vasudevan T. G, Associate Professor, SLS for his support in scientifically editing the manuscript. Our thanks are also due to Mr. Sudhakar Kotian and Mr. Dheeraj for their help in animal handling and care.

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Authors and Affiliations

  1. Department of Biophysics, School of Life Sciences, Manipal University, Manipal, 576104, India

    Bharath Rathnakar, Vijendra Prabhu, Subhash Chandra & Krishna Kishore Mahato

  2. Department of Radiation Biology and Toxicology, School of Life Sciences, Manipal University, Manipal, 576104, India

    Bola Sadashiva Satish Rao

  3. Department of Biotechnology, MIT, Manipal University, Manipal, 576104, India

    Vijendra Prabhu

  4. Department of Pathology, KMC Mangalore, Manipal University, Mangalore, 576104, India

    Sharada Rai

  5. Department of Pathology, KMC Manipal, Manipal University, Manipal, 576104, India

    Anuradha Calicut Kini Rao

  6. Department of Pathology, Srinivas Institute of Medical Sciences and Research Centre, Surathkal, Mangalore, 575021, India

    Anuradha Calicut Kini Rao

  7. Laser Biomedical Applications and Instrumentation Division, RRCAT, Indore, 452013, India

    Mrinalini Sharma & Pradeep Kumar Gupta

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  1. Bharath Rathnakar
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  2. Bola Sadashiva Satish Rao
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Correspondence to Krishna Kishore Mahato.

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Rathnakar, B., Rao, B.S.S., Prabhu, V. et al. Photo-biomodulatory response of low-power laser irradiation on burn tissue repair in mice. Lasers Med Sci 31, 1741–1750 (2016). https://doi.org/10.1007/s10103-016-2044-2

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