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Inhibition of Japanese Encephalitis Virus Infection by the Sulfated Polysaccharide Extracts from Ulva lactuca

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Abstract

Japanese encephalitis virus (JEV), a neurotropic flavivirus, is one of the major causes of acute encephalitis in humans. After infection, inflammatory reactions and neurological diseases often develop. Still there are no effective drugs available against virus infection. Recently, extracts of algae have been shown to possess a broad range of biological activities including antivirus activity. In this study, we identified that the sulfated polysaccharide extracts from Ulva lactuca can inhibit JEV infection in Vero cells. Mechanistic studies further revealed that the Ulva sulfated polysaccharide extracts can block virus adsorption and thus make the virus unable to enter cells. The Ulva sulfated polysaccharide extracts also effectively decrease the production of pro-inflammatory cytokines in the JEV-infected primary mixed glia cells. In an animal study, the JEV-infected C3H/HeN mice appeared to have neurobehavioral abnormalities on the fifth day and died on the seventh day post infection. However, the JEV-infected mice pretreated with the Ulva sulfated polysaccharide extracts can delay the onset of hind limb paralysis and thereby prevent mice from death.

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Acknowledgment

This work was financially supported by the National Science Council, Taiwan, under grant numbers of NSC 98-2627-B-019-005 and NSC 99-2627-B-019-002.

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Authors and Affiliations

  1. Department of Food Science, National Taiwan Ocean University, 2 Pei Ning Road, Keelung, Taiwan

    Ya-Huang Chiu & Chang-Jer Wu

  2. Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan

    Yi-Lin Chan

  3. Genomics Research Center, Academia Sinica, Taipei, Taiwan

    Tsung-Lin Li

  4. Center of Excellence for Marine Bioenvironment and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan

    Chang-Jer Wu

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  1. Ya-Huang Chiu
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  2. Yi-Lin Chan
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Correspondence to Chang-Jer Wu.

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Chiu, YH., Chan, YL., Li, TL. et al. Inhibition of Japanese Encephalitis Virus Infection by the Sulfated Polysaccharide Extracts from Ulva lactuca . Mar Biotechnol 14, 468–478 (2012). https://doi.org/10.1007/s10126-011-9428-x

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