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Thermal analysis of the dentine tubule under hot and cold stimuli using fluid–structure interaction simulation

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Abstract

The objective of this study is to compare the thermal stress changes in the tooth microstructures and the hydrodynamic changes of the dental fluid under hot and cold stimuli. The dimension of the microstructures of eleven cats’ teeth was measured by scanning electron microscopy, and the changes in thermal stress during cold and hot stimulation were calculated by 3D fluid–structure interaction modeling. Evaluation of results, following data validation, indicated that the maximum velocities in cold and hot stimuli were − 410.2 ± 17.6 and + 205.1 ± 8.7 µm/s, respectively. The corresponding data for maximum thermal stress were − 20.27 ± 0.79 and + 10.13 ± 0.24 cmHg, respectively. The thermal stress caused by cold stimulus could influence almost 2.9 times faster than that caused by hot stimulus, and the durability of the thermal stress caused by hot stimulus was 71% greater than that by cold stimulus under similar conditions. The maximum stress was on the tip of the odontoblast, while the stress in lateral walls of the odontoblast and terminal fibril was very weak. There is hence a higher possibility of pain transmission with activation of stress-sensitive ion channels at the tip of the odontoblast. The maximum thermal stress resulted from the cold stimulus is double that produced by the hot stimulus. There is a higher possibility of pain transmission in the lateral walls of the odontoblast and terminal fibril by releasing mediators during the cold stimulation than the hot stimulation. These two reasons can be associated with a greater pain sensation due to intake of cold liquids.

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References

  • Andrew D, Matthews B (2000) Displacement of the contents of dentinal tubules and sensory transduction in intradental nerves of the cat. J Physiol 529:791–802

    Article  Google Scholar 

  • Bleicher F (2014) Odontoblast physiology. Exp Cell Res 325:65–71

    Article  Google Scholar 

  • Borčić J, Antonić R, Muhvić Urek M et al (2007) 3-D stress analysis in first maxillary premolar. Coll Antropol 31:1025–1029

    Google Scholar 

  • Boreak N, Ishihata H, Shimauchi H (2015) A photochemical method for in vitro evaluation of fluid flow in human dentine. Arch Oral Biol 60:193–198

    Article  Google Scholar 

  • Brännström M (1986) The hydrodynamic theory of dentinal pain: sensation in preparations, caries, and the dentinal crack syndrome. J Endod 12:453–457

    Article  Google Scholar 

  • Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D (1997) The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature 389:816–824

    Article  Google Scholar 

  • Cervino G, Fiorillo L, Spagnuolo G, Bramanti E, Laino L, Lauritano F, Cicciù M (2017) Interface between MTA and dental bonding agents: scanning electron microscope evaluation. J Int Soc Prev Community Dent 7:64

    Article  Google Scholar 

  • Charoenlarp P, Wanachantararak S, Vongsavan N, Matthews B (2007) Pain and the rate of dentinal fluid flow produced by hydrostatic pressure stimulation of exposed dentine in man. Arch Oral Biol 52:625–631

    Article  Google Scholar 

  • Chung G, Oh SB (2013) TRP channels in dental pain. Open Pain J 6:31–36

    Article  Google Scholar 

  • Chung G, Jung SJ, Oh SB (2013) Cellular and molecular mechanisms of dental nociception. J Dent Res 92:948–955

    Article  Google Scholar 

  • Ciucchi B, Bouillaguet S, Holz J, Pashley D (1995) Dentinal fluid dynamics in human teeth, in vivo. J Endod 21:191–194

    Article  Google Scholar 

  • Fearnhead RW (1957) Histological evidence for the innervation of human dentine. J Anat 91:267

    Google Scholar 

  • Garcés-Ortíz M, Ledesma-Montes C, Reyes-Gasga J (2015) Scanning electron microscopic study on the fibrillar structures within dentinal tubules of human dentin. J Endod 41:1510–1514

    Article  Google Scholar 

  • Gholampour S (2018) FSI simulation of CSF hydrodynamic changes in a large population of non-communicating hydrocephalus patients during treatment process with regard to their clinical symptoms. PLoS ONE 13:e0196216

    Article  Google Scholar 

  • Gholampour S, Zoorazma G, Shakouri E (2016) Evaluating the effect of dental filling material and filling depth on the strength and deformation of filled teeth. J Dent Mater Tech 5:172–180

    Google Scholar 

  • Gholampour S, Fatouraee N, Seddighi AS, Seddighi A (2017a) Evaluating the effect of hydrocephalus cause on the manner of changes in the effective parameters and clinical symptoms of the disease. J Clin Neurosci 35:50–55

    Article  Google Scholar 

  • Gholampour S, Fatouraee N, Seddighi AS, Seddighi A (2017b) Numerical simulation of cerebrospinal fluid hydrodynamics in the healing process of hydrocephalus patients. J Appl Mech Tech Phys 58:386–391

    Article  MathSciNet  Google Scholar 

  • Hille B (1984) Ionic channels of excitable membrane. Sinauer Associates Inc, Sunderland

    Google Scholar 

  • Hodgkin AL, Huxley AF (1952) A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol 117:500–544

    Article  Google Scholar 

  • Horiuchi H, Matthews B (1973) In-vitro observations on fluid flow through human dentine caused by pain-producing stimuli. Arch Oral Biol 18:275–294

    Article  Google Scholar 

  • Huang GT (2009) Pulp and dentin tissue engineering and regeneration: current progress. Regen Med 4:697–707

    Article  Google Scholar 

  • Huo N, Tang L, Yang Z et al (2010) Differentiation of dermal multipotent cells into odontogenic lineage induced by embryonic and neonatal tooth germ cell-conditioned medium. Stem Cells Dev 19:93–104

    Article  Google Scholar 

  • Ishihata H, Kanehira M, Nagai T, Finger WJ, Shimauchi H, Komatsu M (2009) Effect of desensitizing agents on dentin permeability. Am J Dent 22:143–146

    Google Scholar 

  • Ishihata H, Finger WJ, Kanehira M, Shimauchi H, Komatsu M (2011) In vitro dentin permeability after application of Gluma® desensitizer as aqueous solution or aqueous fumed silica dispersion. J Appl Oral Sci 19:147–153

    Article  Google Scholar 

  • Khatibi Shahidi M, Krivanek J, Kaukua N, Ernfors P, Hladik L, Kostal V, Masich S, Hampl A, Chubanov V, Gudermann T, Romanov RA (2015) Three-dimensional imaging reveals new compartments and structural adaptations in odontoblasts. J Dent Res 94:945–954

    Article  Google Scholar 

  • Kuisma-Kursula P (2017) Accuracy, precision and detection limits of SEM–WDS, SEM–EDS and PIXE in the multielemental analysis of medieval glass. X-Ray Spectrom 29:111–118

    Article  Google Scholar 

  • Lee BM, Jo H, Park G et al (2017) Extracellular ATP induces calcium signaling in odontoblasts. J Dent Res 96:200–207

    Article  Google Scholar 

  • Lin M, Xu F, Lu TJ, Bai BF (2010) A review of heat transfer in human tooth-experimental characterization and mathematical modeling. Dent Mater 26:501–513

    Article  Google Scholar 

  • Lin M, Liu S, Niu L, Xu F, Lu TJ (2011a) Analysis of thermal-induced dentinal fluid flow and its implications in dental thermal pain. Arch Oral Biol 56:846–854

    Article  Google Scholar 

  • Lin M, Luo ZY, Bai BF, Xu F, Lu TJ (2011b) Fluid mechanics in dentinal microtubules provides mechanistic insights into the difference between hot and cold dental pain. PLoS ONE 6:e18068

    Article  Google Scholar 

  • Lin M, Genin GM, Xu F, Lu T (2014) Thermal pain in teeth: electrophysiology governed by thermomechanics. Appl Mech Rev 66:030801

    Article  Google Scholar 

  • Lin M, Liu F, Liu S et al (2017) The race to the nociceptor: mechanical versus temperature effects in thermal pain of dental neurons. Acta Mech Sin 33:260–266

    Article  MathSciNet  Google Scholar 

  • Linsuwanont P, Palamara JE, Messer HH (2007) An investigation of thermal stimulation in intact teeth. Arch Oral Biol 52:218–227

    Article  Google Scholar 

  • Linsuwanont P, Versluis A, Palamara JE, Messer HH (2008) Thermal stimulation causes tooth deformation: a possible alternative to the hydrodynamic theory? Arch Oral Biol 53:261–272

    Article  Google Scholar 

  • Lo Giudice G, Cutroneo G, Centofanti A, Artemisia A, Bramanti E, Militi A, Rizzo G, Favaloro A, Irrera A, Lo Giudice R, Cicciù M (2015) Dentin morphology of root canal surface: a quantitative evaluation based on a scanning electronic microscopy study. BioMed Res Int 27:ID164065

    Google Scholar 

  • Loyd DR, Sun XX, Locke EE, Salas MM, Hargreaves KM (2012) Sex differences in serotonin enhancement of capsaicin-evoked calcitonin gene-related peptide release from human dental pulp. PAIN® 153:2061–2067

    Article  Google Scholar 

  • Matthews B (1977) Responses of intradental nerves to electrical and thermal stimulation of teeth in dogs. J Physiol 264:641–664

    Article  Google Scholar 

  • McKemy DD, Neuhausser WM, Julius D (2002) Identification of a cold receptor reveals a general role for TRP channels in thermosensation. Nature 416:52–58

    Article  Google Scholar 

  • Metzger TA, Niebur GL (2016) Comparison of solid and fluid constitutive models of bone marrow during trabecular bone compression. J Biomech 49:3596–3601

    Article  Google Scholar 

  • Oskui IZ, Ashtiani MN, Hashemi A, Jafarzadeh H (2013) Thermal analysis of the intact mandibular premolar: a finite element analysis. Int Endod J 46:841–846

    Article  Google Scholar 

  • Oskui IZ, Ashtiani MN, Hashemi A, Jafarzadeh H (2014) Effect of thermal stresses on the mechanism of tooth pain. J Endod 40:1835–1839

    Article  Google Scholar 

  • Pashley DH, Nelson R, Pashley EL (1981) In-vivo fluid movement across dentine in the dog. Arch Oral Biol 26:707–710

    Article  Google Scholar 

  • Shibukawa Y, Sato M, Kimura M, Sobhan U, Shimada M, Nishiyama A, Kawaguchi A, Soya M, Kuroda H, Katakura A, Ichinohe T (2015) Odontoblasts as sensory receptors: transient receptor potential channels, pannexin-1, and ionotropic ATP receptors mediate intercellular odontoblast-neuron signal transduction. Pflügers Arch Eur J Physiol 467:843–863

    Article  Google Scholar 

  • Trifkovic B, Budak I, Todorovic A, Hodolic J, Puskar T, Jevremovic D, Vukelic D (2012) Application of replica technique and SEM in accuracy measurement of ceramic crowns. Meas Sci Rev 12:90–97

    Article  Google Scholar 

  • Vongsavan N, Matthews B (1992) Fluid flow through cat dentine in vivo. Arch Oral Biol 37:175–185

    Article  Google Scholar 

  • Vongsavan N, Matthews B (2007) The relationship between the discharge of intradental nerves and the rate of fluid flow through dentine in the cat. Arch Oral Biol 52:640–647

    Article  Google Scholar 

  • Wang J, Jin X, Ma P (2014) Dentin–pulp tissue engineering and regeneration. In: Ma P (ed) Biomaterials and regenerative medicine. Cambridge University, Cambridge, pp 570-582

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biomedical Engineering, Islamic Azad University-North Tehran Branch, P.O.B. 1651153311, Tehran, Iran

    Seifollah Gholampour & Amin Jalali

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  1. Seifollah Gholampour
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  2. Amin Jalali
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Correspondence to Seifollah Gholampour.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of Shohada Tajrish Hospital research committee North Tehran Branch, Islamic Azad University, Tehran, Iran (Ethics committee of biomedical research center) and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Gholampour, S., Jalali, A. Thermal analysis of the dentine tubule under hot and cold stimuli using fluid–structure interaction simulation. Biomech Model Mechanobiol 17, 1599–1610 (2018). https://doi.org/10.1007/s10237-018-1046-3

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  • DOI: https://doi.org/10.1007/s10237-018-1046-3

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