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Induction of defense responses in pea tissues by inorganic phosphate

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Abstract

When inorganic phosphate, a common and essential element for organisms, was applied endogenously, a rejection reaction and superoxide generation were induced in pea tissues but phytoalexin production was not. Phosphate-induced superoxide generation was sensitive to cycloheximide (CHX) and salicylhydroxamic acid (SHAM), indicating that part of the generation was dependent upon the expression of peroxidase gene(s). Peroxidases (POXs) are well known not only to scavenge hydrogen peroxide with phenolics but also to generate superoxide via NADH oxidation in the presence of p-coumaric acid and manganese ion. We cloned five pea POX cDNAs that are predicted to be located outside of the cells. The accumulation of five POX mRNAs, NTPase mRNA, and phenylalanine ammonia-lyase mRNA was measured by semiquantitative reverse transcription-polymerase chain reaction. The expression of the five POX genes was induced by a fungal elicitor. On the other hand, inorganic phosphate induced the accumulation of POX11, POX14, and POX21 mRNAs but not of POX13, POX29, and PsPAL1 mRNAs within 1–3 h after treatment of pea seedlings. In view of these findings, we discuss inorganic phosphate as a signal transmitter inducing part of the plant defense responses.

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References

  • JR Aist H Kunoh HW Israel (1979) ArticleTitleChallenge appressoria of Erysiphe graminis fail to breach preformed papillae of a compatible barley cultivar Phytopathology 69 1245–1250 Occurrence Handle10.1094/Phyto-69-1245

    Article  Google Scholar 

  • S Bohman J Staal BPHJ Thomma M Wang C Dixelius (2004) ArticleTitleCharacterisation of an Arabidopsis-Leptosphaeria maculans pathosystem: resistance partially requires camalexin biosynthesis and is independent of salicylic acid, ethylene and jasmonic acid signalling Plant J 37 9–20 Occurrence Handle1:CAS:528:DC%2BD2cXptFKmsw%3D%3D Occurrence Handle10.1046/j.1365-313X.2003.01927.x Occurrence Handle14675428

    Article  CAS  PubMed  Google Scholar 

  • MM Bradford (1976) ArticleTitleA rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248–254 Occurrence Handle1:CAS:528:DyaE28XksVehtrY%3D Occurrence Handle10.1016/0003-2697(76)90527-3 Occurrence Handle942051

    Article  CAS  PubMed  Google Scholar 

  • P Chomczynski N Sacchi (1987) ArticleTitleSingle-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Anal Biochem 162 156–159 Occurrence Handle1:CAS:528:DyaL2sXitFSns7Y%3D Occurrence Handle10.1016/0003-2697(87)90021-2 Occurrence Handle2440339

    Article  CAS  PubMed  Google Scholar 

  • RB Day CB McAlvin JT Loh RL Denny TC Wood ND Young G Stacey (2000) ArticleTitleDifferential expression of two soybean apyrases, one of which is an early nodulin Mol Plant Microbe Interact 13 1053–1070 Occurrence Handle1:CAS:528:DC%2BD3cXntVarsrw%3D Occurrence Handle11043467

    CAS  PubMed  Google Scholar 

  • ME Etzler G Kalsi NN Ewing NJ Roberts RB Day JB Murphy (1999) ArticleTitleA nod factor binding lectin with apyrase activity from legume roots Proc Natl Acad Sci USA 96 5856–5861 Occurrence Handle1:CAS:528:DyaK1MXjtFCnu7k%3D Occurrence Handle10.1073/pnas.96.10.5856 Occurrence Handle10318974

    Article  CAS  PubMed  Google Scholar 

  • BJ Feys JE Parker (2000) ArticleTitleInterplay of signaling pathways in plant disease resistance Trends Genet 16 449–455 Occurrence Handle1:CAS:528:DC%2BD3cXotVSrsLc%3D Occurrence Handle10.1016/S0168-9525(00)02107-7 Occurrence Handle11050331

    Article  CAS  PubMed  Google Scholar 

  • HD Gottstein JA Kuć (1989) ArticleTitleInduction of systemic resistance to anthracnose in cucumber by phosphates Phytopathology 79 176–179

    Google Scholar 

  • T Kawahara K Toyoda A Kiba A Miura T Ohgawara M Yamamoto Y Inagaki Y Ichinose T Shiraishi (2003) ArticleTitleCloning and characterization of pea apyrases: involvement of PsAPY1 in response to signal molecules from the pea pathogen Mycosphaerella pinodes J Gen Plant Pathol 69 33–38 Occurrence Handle1:CAS:528:DC%2BD3sXjtVKktro%3D Occurrence Handle10.1007/s10327-002-0003-1

    Article  CAS  Google Scholar 

  • A Kiba K Toyoda T Yamada Y Ichinose T Shiraishi (1995) ArticleTitleSpecific inhibition of cell wall-bound ATPases by fungal suppressor from Mycosphaerella pinodes Plant Cell Physiol 36 809–817 Occurrence Handle1:CAS:528:DyaK2MXntlagsrg%3D

    CAS  Google Scholar 

  • A Kiba K Toyoda Y Ichinose T Yamada T Shiraishi (1996a) ArticleTitleSpecific response of partially purified cell wall-bound ATPases to fungal suppressor Plant Cell Physiol 37 207–214 Occurrence Handle1:CAS:528:DyaK28XhvVGisro%3D

    CAS  Google Scholar 

  • A Kiba K Toyoda Y Ichinose T Yamada T Shiraishi (1996b) ArticleTitleSpecies-specific suppression of superoxide-anion generation on surfaces of pea leaves by the suppressor from Mycospaerella pinodes Ann Phytopathol Soc Jpn 62 508–512 Occurrence Handle1:CAS:528:DyaK28XmvVKqtL0%3D

    CAS  Google Scholar 

  • A Kiba C Miyake K Toyoda T Yamada Y Ichinose T Shiraishi (1997) ArticleTitleSuperoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules Phytopathology 87 846–852 Occurrence Handle1:CAS:528:DyaK2sXlsFCqtbo%3D Occurrence Handle18945053

    CAS  PubMed  Google Scholar 

  • SR Long (1996) ArticleTitle Rhizobium symbiosis: nod factor in perspective Plant Cell 8 1885–1898 Occurrence Handle1:CAS:528:DyaK28Xmsl2mu7o%3D Occurrence Handle10.1105/tpc.8.10.1885 Occurrence Handle8914326

    Article  CAS  PubMed  Google Scholar 

  • M Matsubara H Kuroda (1987) ArticleTitleThe structure and physiological activity of a glycoprotein secreted from conidia of Mycosphaerella pinodes. II Chem Pharm Bull 35 249–255 Occurrence Handle1:CAS:528:DyaL2sXhsFaltbo%3D Occurrence Handle3594654

    CAS  PubMed  Google Scholar 

  • K Nakai M Kanehisa (1991) ArticleTitleExpert system for predicting protein localization sites in Gram-negative bacteria Proteins 11 95–110 Occurrence Handle1:CAS:528:DyaK38XhtVaitLg%3D Occurrence Handle10.1002/prot.340110203 Occurrence Handle1946347

    Article  CAS  PubMed  Google Scholar 

  • DG Nathan RL Baehner DK Weaver (1969) ArticleTitleFailure of nitroblue tetrazolium reduction in the phagocytic vacuoles of leukocytes in chronic granulomatous disease J Clin Invest 48 1895–1904 Occurrence Handle1:CAS:528:DyaE3cXhs1ah Occurrence Handle5387730 Occurrence Handle10.1172/JCI106156

    Article  CAS  PubMed  Google Scholar 

  • NJ Roberts J Brigham B Wu JB Murphy H Volpin DA Phillips ME Etzler (1999) ArticleTitleA nod factor-binding lectin is a member of a distinct class of apyrases that may be unique to the legumes Mol Gen Genet 262 261–267 Occurrence Handle1:CAS:528:DyaK1MXntFCksbc%3D Occurrence Handle10.1007/s004380051082 Occurrence Handle10517321

    Article  CAS  PubMed  Google Scholar 

  • KR Senthil H-M Peng DR Cook (2002) ArticleTitleNod factor induction of reactive oxygen species production is correlated with expression of the early nodulin gene rip1 in Medicago truncatula Mol Plant Microbe Interact 15 522–528

    Google Scholar 

  • T Shiraishi H Oku M Yamashita S Ouchi (1978) ArticleTitleElicitor and suppressor of pisatin induction in spore germination fluid of pea pathogen, Mycosphaerella pinodes Ann Phytopathol Soc Jpn 44 659–665

    Google Scholar 

  • T Shiraishi K Saitoh H-M Kim T Kato M Tahara H Oku T Yamada Y Ichinose (1992) ArticleTitleTwo suppressors, supprescin A and B, secreted by a pea pathogen, Mycosphaerella pinodes Plant Cell Physiol 33 663–667 Occurrence Handle1:CAS:528:DyaK38XlvVOqsLw%3D

    CAS  Google Scholar 

  • T Shiraishi T Yamada Y Ichinose A Kiba K Toyoda (1997) ArticleTitleThe role of suppressors in determining host-parasite specificities in plant cells Int Rev Cytol 172 55–93 Occurrence Handle1:CAS:528:DyaK2sXivFemsrc%3D Occurrence Handle10.1016/S0074-7696(08)62358-X

    Article  CAS  Google Scholar 

  • K Toyoda T Shiraishi H Yoshioka T Yamada Y Ichinose H Oku (1992) ArticleTitleRegulation of polyphosphoinositide metabolism in pea plasma membranes by elicitor and suppressor from a pea pathogen, Mycosphaerella pinodes Plant Cell Physiol 33 445–452 Occurrence Handle1:CAS:528:DyaK38Xks1Kkuro%3D

    CAS  Google Scholar 

  • T Yamada H Hashimoto T Shiraishi H Oku (1989) ArticleTitleSuppression of pisatin, phenylalanine ammonia-lyase mRNA, and chalcone synthase mRNA by putative pathogenicity factor from the fungus Mycosphaerella pinodes Mol Plant Microbe Interact 2 256–261

    Google Scholar 

  • Y Yamamoto H Oku T Shiraishi S Ouchi K Koshizawa (1986) ArticleTitleNon-specific induction of pisatin and local resistance in pea leaves by elicitor from Mycosphaerella pinodes, M. melonis and M. ligulicola and effect of suppressor from M. pinodes J Phytopathology 117 136–143 Occurrence Handle1:CAS:528:DyaL2sXjtVWltA%3D%3D

    CAS  Google Scholar 

  • H Yoshioka T Shiraishi S Kawamata K Nasu T Yamada Y Ichinose H Oku (1992) ArticleTitleOrthovanadate suppresses accumulation of phenylalanine ammonia-lyase mRNA and chalcone synthase mRNA in pea epicotyls induced by elicitor from Mycosphaerella pinodes Plant Cell Physiol 33 201–204 Occurrence Handle1:CAS:528:DyaK38XitFWhsbg%3D

    CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University, Okayama, 700-8530, Japan

    Tomoharu Kawahara, Hiroko Namba, Kazuhiro Toyoda, Tomonari Kasai, Megumi Sugimoto, Yoshishige Inagaki, Yuki Ichinose & Tomonori Shiraishi

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  1. Tomoharu Kawahara
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  2. Hiroko Namba
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  3. Kazuhiro Toyoda
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  4. Tomonari Kasai
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  5. Megumi Sugimoto
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  6. Yoshishige Inagaki
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  7. Yuki Ichinose
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  8. Tomonori Shiraishi
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Corresponding author

Correspondence to Tomonori Shiraishi.

Additional information

The nucleotide sequence data for PsPOX11, 13, 14, 21, and 29 reported are available in the DDBJ database under accession numbers AB193816, AB193817, AB193818, AB193819, and AB193820, respectively.

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Kawahara, T., Namba, H., Toyoda, K. et al. Induction of defense responses in pea tissues by inorganic phosphate. J Gen Plant Pathol 72, 129–136 (2006). https://doi.org/10.1007/s10327-005-0261-9

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  • DOI: https://doi.org/10.1007/s10327-005-0261-9

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