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N605ab, a specific molecular marker for Phytophthora infestans, distinguishes three genotypes in Japan

  • Fungal Diseases
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Abstract

Random amplified polymorphic DNA (RAPD) analysis using the OPG-06 primer generated specific patterns for Japanese genotypes US-1, JP-1, and a new A1 (JP-2, JP-3, and JP-4) of Phytophthora infestans. N605, a specific RAPD fragment, was cloned and sequenced. PCR primers BD1/BD2 were constructed based on the N605 sequence and were used to clarify the genotypes. PCR products using the BD1/BD2 primers (N605ab marker) easily distinguished the new A1 from US-1 and JP-1. This technique provides a simple and effective method for rapid genotype discrimination that can be used in ecological experiments and forecasts for the occurrence of late blight.

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Author notes

  1. Yuko Shirasawa

    Present address: Food and Agricultural Materials Inspection Center, Odori Nishi 10-4-1, Sapporo, 060-0042, Japan

Authors and Affiliations

  1. Laboratory of Plant Pathology, Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan

    Seishi Akino, Yuko Shirasawa, Norio Kondo & Shigeo Naito

Authors
  1. Seishi Akino
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  2. Yuko Shirasawa
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  3. Norio Kondo
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  4. Shigeo Naito
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Correspondence to Seishi Akino.

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Akino, S., Shirasawa, Y., Kondo, N. et al. N605ab, a specific molecular marker for Phytophthora infestans, distinguishes three genotypes in Japan. J Gen Plant Pathol 74, 125–127 (2008). https://doi.org/10.1007/s10327-007-0065-1

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  • DOI: https://doi.org/10.1007/s10327-007-0065-1

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