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Foliar spray of a cell wall protein fraction from the biocontrol agent Pythium oligandrum induces defence-related genes and increases resistance against Cercospora leaf spot in sugar beet

  • Fungal Diseases
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Abstract

After a cell wall protein fraction (CWP) of Pythium oligandrum was sprayed on sugar beet leaves, we screened leaves for induced expression of defence-related genes and for resistance against Cercospora leaf spot. In a western blot analysis, the CWP was primarily retained on the surface of leaves without degradation for at least 48 h after spraying. In northern blot analyses, four defence-related genes (β-1, 3-glucanase, acidic class III chitinase, 5-enol-pyruvylshikimate-phosphate synthase and oxalate oxidase-like germin) were expressed more rapidly in CWP-treated leaves compared to control leaves treated with distilled water (DW). When CWP was applied to a suspension of cultured cells of sugar beet, an oxidative burst was observed that did not occur after the DW treatment. In growth chamber trials after inoculation with Cercospora beticola, the severity of Cercospora leaf spot was significantly reduced in CWP-treated plants compared to the DW-treated controls. In a field experiment, CWP treatment was also effective against the disease. CWP did not reduce growth rate of the pathogen in plate tests. The results together suggest that the CWP from P. oligandrum can be retained on the leaf surface and induce expression of disease resistance genes, thereby reducing Cercospora leaf spot on sugar beet.

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Acknowledgments

This research was supported by a Grant-in-Aid (Development of new biorational techniques for sustainable agriculture) from the Ministry of Agriculture, Forestry and Fisheries, Japan. We thank the members of Biomass Research Team of National Agricultural Research Center of Hokkaido Region for generous gift of sugar beet line NK-219 mm-0 and for support of the field test. We also thank Atsuko Muraki and Miyuki Yamaguchi for their technical assistance.

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  1. Shigehito Takenaka

    Present address: Department of Research Planning and Coordination, Headquarters National Agriculture and Food Research Organization, 3-1-1 Kannoidai, Tsukuba, Ibaraki, 305-8517, Japan

Authors and Affiliations

  1. Memuro Research Station, National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro-cho, Kasaigun, Hokkaido, 082-0081, Japan

    Shigehito Takenaka

  2. Plant Genetic Resources Division, Hokkaido Central Agricultural Experiment Station, Minamitakinogawa, Takikawa, Hokkaido, 073-0013, Japan

    Hideto Tamagake

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  1. Shigehito Takenaka
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Correspondence to Shigehito Takenaka.

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Takenaka, S., Tamagake, H. Foliar spray of a cell wall protein fraction from the biocontrol agent Pythium oligandrum induces defence-related genes and increases resistance against Cercospora leaf spot in sugar beet. J Gen Plant Pathol 75, 340–348 (2009). https://doi.org/10.1007/s10327-009-0186-9

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