Abstract
To detect and count DNA double-strand breaks (DSBs) during the life cycle of Magnaporthe oryzae, we constructed an expression vector for EGFP-Rhm51 fusion protein and introduced it into strain Ina86-137. EGFP-Rhm51 foci were detected, and the number of foci in mycelia treated with mitomycin C, which induces DSBs, was higher than in untreated samples, indicating that the foci enabled the visualization of DSBs during the life cycle. EGFP-Rhm51 foci were observed at all stages of the asexual cycle including when invasive hyphae formed in an intact rice leaf sheath, demonstrating that M. oryzae undergoes DSBs during vegetative and parasitic growth.
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Abbreviations
- DSBs:
-
Double-strand breaks
- Hr:
-
Homologous recombination
- ORF:
-
Open reading frame
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Acknowledgments
The authors are grateful to the Islamic Development Bank for supporting Ndindeng’s study at Hokkaido University through the Merit Scholarship Programme for High Technology (MSP).
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Ndindeng, S.A., Miki, S., Abe, A. et al. EGFP-Rhm51 foci enable the visualization and enumeration of DNA double-strand breaks in Magnaporthe oryzae . J Gen Plant Pathol 76, 377–381 (2010). https://doi.org/10.1007/s10327-010-0271-0
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DOI: https://doi.org/10.1007/s10327-010-0271-0