Abstract
Glycosylation of flagellin is known to be involved in filament stabilization, motility, and virulence in Pseudomonas syringae. Here we investigated flagellin glycosylation in other phytopathogenic bacteria. Analyses of deduced amino acid sequences, glycostaining, and molecular masses of purified flagellins revealed that flagellins from all phytopathogenic bacteria investigated were glycosylated. Furthermore, the flagellin in a glycosylation-defective mutant of Xanthomonas campestris pv. campestris (Xcc) had a reduced molecular mass, and motility and virulence of the mutant toward host leaves decreased. These results suggest that flagellin glycosylation is ubiquitous in most phytopathogenic bacteria and that flagellin glycosylation is required for virulence in Xcc.
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Acknowledgments
We thank Emeritus Prof. S. Tsuyumu, Shizuoka University, Prof. Y. Hikichi, Kochi University, and NIAS Genebank for providing Pectobacterium carotovorum subsp. carotovorum EC1, Burkholderia glumae Pg-10 and Pseudomonas cichorii KN52, and all other bacteria investigated in this study. This work was supported in part by Technology of Japan and the Program for Promotion of Basic Research Activities for Innovative Bioscience (PROBRAIN).
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The nucleotide sequences of the fliC and fgt genes have been deposited in the DDBJ, EMBL, and GenBank nucleotide sequence databases under accession numbers AB775209 to AB775214.
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Ichinose, Y., Taguchi, F., Yamamoto, M. et al. Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria. J Gen Plant Pathol 79, 359–365 (2013). https://doi.org/10.1007/s10327-013-0464-4
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DOI: https://doi.org/10.1007/s10327-013-0464-4