Skip to main content
SpringerLink
Log in

Chemical composition and bioactivities of three Chrysanthemum essential oils against Tribolium confusum (du Val) (Coleoptera: Tenebrionidae)

  • Original Paper
  • Published:
Journal of Pest Science Aims and scope Submit manuscript

Abstract

Essential oils from three species of Chrysanthemum growing in Tunisia (C. coronarium, C. fuscatum, and C. grandiflorum) were first analyzed by gas chromatography (GC) and GC/mass spectrometry (MS) and then evaluated for toxicity and antifeeding action against Tribolium confusum. The essential oils obtained from leaves and flowers shared a similar qualitative composition, but the relative proportions of the constituents were quite different. The essential oil of C. grandiflorum was rich in sesquiterpenoids, while those of C. fuscatum and C. coronarium were rich in monoterpenoids. The main common constituents of all the essential oils were α-pinene, myrcene, α-humulene, β-caryophylene, spathulenol, and caryophyllene oxide. The most effective essential oil was obtained from the leaves of C. grandiflorum, that inhibited the relative growth rate (−0.03 mg/mg/d), efficiency of conversion of ingested food (−50.69%), relative consumption rate, caused an antifeeding effect (66.43%) and a high mortality (80%) of T. confusum larvae. Topical application C. grandiflorum essential oil caused a significant insect mortality that attends 27% after 7 days of treatment. While essential oil from C. coronarium flowers has contact and fumigant toxicity with a mortality of 9 and 13%, respectively. Results analysis highlights a relationship between essential oils composition and insecticidal activity against T. confusum. The study showed that each essential oil has specific chemical composition and act differently according to the nature of attributed test. The use of essential oils from different Chrysanthemum species with different methods helps poor farmers who store small amounts of grains to preserve it against pest infestation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    CAS  Google Scholar 

  • Abubakar MS, Abdurahman EM, Haruna AK (2000) The repellant and antifeedant proprieties of Cyperrus articulatus against Tribolium castanenum Hbst. Phytother Res 14:281–283

    Article  PubMed  CAS  Google Scholar 

  • Adams RP (1995) Identification of essential oil components by gas chromatography mass spectroscopy. Allured Publ Corp, Carol Stream

    Google Scholar 

  • Adarkwah C, Obeng-Ofori D, Büttner C, Reichmuth C, Schöller M (2010) Bio-rational control of red flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) in stored wheat with Calneem® oil derived from neem seeds. J Pest Sci 83:471–479

    Article  Google Scholar 

  • Ahn YI, Lee SB, Lee HS, Kim GH (1998) Insecticidal and acaricidal activity of caravacrol and (-thujaplicine derived from Thujopsis dolabrata var. hondai sawdust. J Chem Ecol 24:1–90

    Article  Google Scholar 

  • Aitken AD (1975) Insect travelers, I: Coleoptera. technical bulletin 31, H.M.S.O, London

  • Alvarez-Castellanos PP, Pascual-Villalobos MJ (2003) Effect of fertilizer on yield and composition of flowerhead essential oil of Chrysanthemum coronarium (Asteraceae) cultivated in Spain. Ind Crop Prod 17:77–81

    Article  CAS  Google Scholar 

  • Arthur FH (1996) Grain protectants: current status and prospects for the future. J Stored Prod Res 32:293–302

    Article  Google Scholar 

  • Boussalis AM, Ferraro EG, Martino VS, Pinzón Coussio DJ, Alvarez JC (1999) Argentine plants as potential source of insecticidal compound. J Ethnopharmacol 67:219–223

    Article  Google Scholar 

  • Choi WS, Park BS, Lee YH, Jang DY, Yoon HY, Lee SE (2006) Fumigant toxicities of essential oils and monoterpenes against Lycoriella mali adults. Crop Prot 25:398–401

    Article  CAS  Google Scholar 

  • Coats JR, Karr LL, Drewes CD (1991) Toxicity and neurotoxic effects of monoterpenoids in insects and earthworms. In: Hedin PA (ed) Naturally occurring pest bioregulators. ACS symposium series No. 449. American Chemical Society, Washington, DC, pp 305–316

  • Davies NW (1990) Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and carbowax 20 M phases. J Chromatogr 503:1–24

    Article  CAS  Google Scholar 

  • De-Oliveira AC, Ribeiro-Pinto LF, Paumgartten JR (1997) In vitro inhibition of CYP2B1 monooxygenase by myrcene and other monoterpenoid compounds. Toxicol Lett 92:39–46

    Article  PubMed  CAS  Google Scholar 

  • Enan E (2001a) Insecticidal activity of essential oils: octopaminergic sites of action. Comp Biochem Physiol 130:325–327

    CAS  Google Scholar 

  • Enan EE (2001) Insecticidal activity of essential oils: octopaminergic sites of action. The ESA 2001 annual meeting: an entomological odyssey of ESA. San Diego

  • Farrar RR, Barbour JD, Kenedy GG (1989) Quantifying food consumption and growth in insects. Ann Entomol Soc Am 82:593–598

    Google Scholar 

  • Fields PG, Xie YS, Hou X (2001) Repellent effect of pea (Pisum sativum) fractions against stored-product insects. J Stored Prod Res 37:359–370

    Article  PubMed  Google Scholar 

  • Flamini G, Cioni PL, Morelli I (2003) Differences in the fragrances of pollen, leaves, and floral parts of garland (Chrysanthemum coronarium) and composition of the essential oils from flowerheads and leaves. J Agric Food Chem 51:2267–2271

    Article  PubMed  CAS  Google Scholar 

  • Floc’h E, Boulos L (2008) Flore de Tunisie. Catalogue synonymique commenté, Montpellier

    Google Scholar 

  • Huang Y, Tan JMWL, Kini RM, Ho SH (1997) Toxic and antifeedant action of nutmeg oil against Tribolium castaneum (Herbst) and Sitophilus zeamais Motsch. J Stored Prod Res 33:289–298

    Article  CAS  Google Scholar 

  • Huang H, Lam SL, Ho SH (2000) Bioactivities of essential oil from Elletaria cardamomum (L.) Maton. to Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst). J Stored Prod Res 36:107–117

    Article  CAS  Google Scholar 

  • Isman MB (2000) Plant essential oils for pest and disease management. Crop Prot 19:603–608

    Article  CAS  Google Scholar 

  • Isman MB (2006) Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annu Rev Entomol 51:45–66

    Article  PubMed  CAS  Google Scholar 

  • Jennings W, Shibamoto T (1980) Qualitative analysis of flavor and fragrance volatiles by glass capillary chromatography. Academic Press, New York

    Google Scholar 

  • Lee BH, Choi WS, Lee SE, Park BS (2001) Fumigant toxicity of essential oils and their constituent compounds towards the rice weevil Sitophilus oryzae (L.). Crop Prot 20:317–320

    Article  CAS  Google Scholar 

  • Lee S, Peterson CJ, Coats JR (2002) Fumigation toxicity of monoterpenoids to several stored product insects. J Stored Prod Res 39:77–85

    Article  Google Scholar 

  • Manuwoto S, Scriber JM (1982) Consumption and utilization of three maize genotypes by the southern armyworm. J Econ Entomol 75:163–167

    CAS  Google Scholar 

  • Massada Y (1976) Analysis of essential oils by gas chromatography and mass spectrometry. Wiley, New York

    Google Scholar 

  • Navia-Giné WG, Yuan JS, Mauromoustakos A, Murphy JB, Chen F, Korth KL (2009) Medicago truncatula (E)-β-ocimene synthase is induced by insect herbivory with corresponding increases in emission of volatile ocimene. Plant Physiol Biochem 47:416–425

    Article  PubMed  Google Scholar 

  • Nenaah G (2011) Individual and synergistic toxicity of solanaceous glycoalkaloid against two coleopteran stored-product insects. J Pest Sci 84:77–86

    Article  Google Scholar 

  • Pascual-Villalobos MJ, Fernández M (1999) Insecticidal activity of ethanolic extracts of Urginea maritima (L.) Baker bulbs. Ind Crop Prod 10:115–120

    Article  Google Scholar 

  • Pottier-Alapetite G (1981) La flore de la Tunisie:Angiosperme-Dicotylédones Gamopétales publié par Ministère de l’Enseignement Supérieur et de la Recherche Scientifique et le Ministère de l’Agriculture. Tunis, pp 939–1007

  • Pungitore CR, Garcıa M, Gianello JC, Sosa ME, Tonn CE (2005) Insecticidal and antifeedant effects of Junellia aspera (Verbenaceae) triterpenes and derivatives on Sitophilus oryzae (Coleoptera: Curculionidae). J Stored Prod Res 41:433–443

    Article  CAS  Google Scholar 

  • Rees DP (1995) Coleoptera. In: Subramanyam Bh, Hagstrum DW (eds) Integrated management of insects in stored products. Marcel Dekker, New York, pp 1–39

    Google Scholar 

  • Regnault-Roger C, Hamraoui A (1995) Fumigant toxic activity and reproductive inhibition induced by monoterpenes on Aeanthoseelides obteetus (Say) (Coleoptera), a bruchid of kidney bean (Phaseolus vulgaris L.). J Stored Prod Res 31:291–299

    Article  CAS  Google Scholar 

  • Ryan MF, Byrne O (1988) Plant insect coevolution and inhibition of acetylcholinesterase. J Chem Ecol 14:1965–1975

    Article  CAS  Google Scholar 

  • Schoonhoven LM, van Loon JJA, Dicke M (2005) Insect-plant biology. University Press, Oxford

    Google Scholar 

  • Shaaya E, Kostjukovski M, Eilberg J, Sukprakarn C (1997) Plant oils as fumigants and contact insecticides for the control of stored-product insects. J Stored Prod Res 33(1):7–15

    Article  CAS  Google Scholar 

  • Shunying Z, Yang Y, Huaidong Y, Yue Y, Guolin Z (2005) Chemical composition and antimicrobial activity of the essential oils of Chrysanthemum indicum. J Ethnopharmacol 96:151–158

    Article  PubMed  Google Scholar 

  • Stamopoulos DC, Damos P, Karagianidou G (2007) Bioactivity of five monoterpenoid vapours to Tribolium confusum (du Val) (Coleoptera: Tenebrionidae). J Stored Prod Res 43:571–577

    Article  CAS  Google Scholar 

  • Stenhagen E, Abrahamsson S, McLafferty FW (1974) Registry of mass spectral data. Wiley, New York

    Google Scholar 

  • Swigar AA, Silverstein RM (1981) Monoterpenes. Aldrich Chemical Company, Milwaukee

    Google Scholar 

  • Taghizadeh Saroukolai A, Moharramipour S, Meshkatalsadat MH (2010) Insecticidal properties of Thymus persicus essential oil against Tribolium castaneum and Sitophilus oryzae. J Pest Sci 83:3–8

    Article  Google Scholar 

  • Tripathii AK, Prajarati V, Verm N, Baiil JR, Bansal RP, Kiianuja SPS, Kumar S (2002) Bioactivities of the leaf essential oil of Curcuma Longa (Var. ch66) on three species of stored-product beetles (Coleoptera). J Econ Entomol 95(1):183–189

    Article  Google Scholar 

  • Uchio Y, Tomosue K, Nakayama M, Yamamura A, Waki T (1981) Constituents of the essential oils from three tetraploid species of Chrysanthemum. Phytochem 20(12):2691–2693

    Article  CAS  Google Scholar 

  • Wittstock U, Gershenzon J (2002) Constitutive plant toxins and their role in defense against herbivores and pathogens. Curr Opin Plant Biol 5:300–307

    Article  PubMed  CAS  Google Scholar 

  • Xie YS, Bodnaryk RP, Fields PG (1996) A rapid and simple flour disk bioassay for testing natural substances active against stored-product insects. Can Entomol 128:865–875

    Article  Google Scholar 

Download references

Acknowledgments

We are grateful to the International Foundation for Science (IFS grant No. F/3968-1) and to the Organisation for the Prohibition of Chemical Weapons (OPCW) for financial support. We thank also, Dr. Fethia Skhiri-Harzallah for her help in Chrysanthemum identification species.

Author information

Authors and Affiliations

  1. Unité de Recherche: invertébrés, micro-organismes et malherbes nuisibles: méthodes alternatives de lutte, Institut Supérieur Agronomique de Chott Mariem, University of Sousse, 4042, Sousse, Tunisia

    Dalila Haouas, Monia Ben Halima-Kamel & Mohamed Habib Ben Hamouda

  2. Dipartimento di Scienze Farmaceutiche, Sede Chimica Bioorganica e Biofarmacia, Università di Pisa, Via Bonanno 33, 56126, Pisa, Italy

    Pier Luigi Cioni & Guido Flamini

Authors
  1. Dalila Haouas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pier Luigi Cioni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Monia Ben Halima-Kamel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guido Flamini
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mohamed Habib Ben Hamouda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dalila Haouas.

Additional information

Communicated by C. G. Athanassiou.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Haouas, D., Cioni, P.L., Ben Halima-Kamel, M. et al. Chemical composition and bioactivities of three Chrysanthemum essential oils against Tribolium confusum (du Val) (Coleoptera: Tenebrionidae). J Pest Sci 85, 367–379 (2012). https://doi.org/10.1007/s10340-012-0420-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10340-012-0420-7

Keywords

Search

Navigation