Abstract
Thirty one pears genotypes from east blacksea region were evaluated for genetic relationships by using Randomly Amplified Polymorphic DNA (RAPD) and ISSR (Inter-Simple Sequence Repeats) markers from total 70 RAPD and ISSR primer investigated, 22 could amplify clearly and consistently. Cluster analysis of the pears genotypes was performed based on data from polymorphic bands RAPD and ISSR by using Jaccard’s similarity coefficient and the Unweighted pair-group method with arithmetic average (UPGMA) clustering method. The 31 pear genotypes were classified into two major groups. Cluster A was divided into 2 subclusters: Gumushane pears and Trabzon pears. Cluster B consisted of Artvin pears. The similarity matrix values ranged between 0.105 and 0.968.
Zusammenfassung
Bei 31 Birnen-Genotypen aus der Ost-Schwarzmeerregion wurde die genetische Verwandtschaft mit Hilfe der RAPD- (Randomly Amplified Polymorphic DNA) und ISSR-Markern (Inter-Simple Sequence Repeats) bestimmt. Von insgesamt 70 überprüften RAPD- und ISSR-Primern führten 22 zu einem eindeutigen und einheitlichen Ergebnis. Mit den Daten der RAPD- und der ISSR-Methode wurde eine Clusteranalyse zu den Birnen-Genotypen durchgeführt (Jaccard Koeefizient und Unweighted pair-group method with arithmetic average (kurz UPGMA)). Die 31 Birnen-Genotypen wurden in zwei Hauptgruppen eingeteilt. Gruppe A wurde in zwei Untergruppen unterteilt, in Birnen aus Gumushane und Birnen aus Trabzon. Die Birnen aus Artvin befanden sich in Gruppe B. Das Ähnlichkeitsmaß lag zwischen 0,105 und 0,968.
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Kalkışım, O., Okcu, M., Okcu, Z. et al. Relationships Among some Pears Genotypes (Pyrus Communis L.) Based on ISSR and RAPD Analysis. Erwerbs-Obstbau 58, 259–264 (2016). https://doi.org/10.1007/s10341-016-0287-5
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DOI: https://doi.org/10.1007/s10341-016-0287-5