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Lipopeptides from Bacillus strain AR2 inhibits biofilm formation by Candida albicans

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Abstract

The ability of the human fungal pathogen Candida albicans to reversibly switch between different morphological forms and establish biofilms is crucial for establishing infection. Targeting phenotypic plasticity and biofilm formation in C. albicans represents a new concept for antifungal drug discovery. The present study evaluated the influence of cyclic lipopeptide biosurfactant produced by Bacillus amyloliquefaciens strain AR2 on C. albicans biofilms. The biosurfactant was characterized as a mixture of iturin and fengycin by MALDI-TOF and amino acid analysis. The biosurfactant exhibited concentration dependent growth inhibition and fungicidal activity. The biosurfactant at sub-minimum growth inhibition concentration decreased cell surface hydrophobicity, hindered germ tube formation and reduced the mRNA expression of hyphae-specific gene HWP1 and ALS3 without exhibiting significant growth inhibition. The biosurfactants inhibited biofilm formation in the range of 46–100 % depending upon the concentration and Candida strains. The biosurfactant treatment dislodged 25–100 % of preformed biofilm from polystyrene plates. The biosurfactant retained its antifungal and antibiofilm activity even after exposure to extreme temperature. By virtue of the ability to inhibit germ tube and biofilm formation, two important traits of C. albicans involved in establishing infection, lipopeptides from strain AR2 may represent a potential candidate for developing heat stable anti-Candida drugs.

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Acknowledgments

The authors thank the Director, IMTECH for providing the facilities for this work. AKS and RR are thankful to UGC and CSIR for fellowship, respectively.

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Authors and Affiliations

  1. Institute of Microbial Technology, Sector 39 A, Chandigarh, 160036, India

    Ria Rautela, Anil Kumar Singh, Abha Shukla & Swaranjit Singh Cameotra

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  1. Ria Rautela
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  2. Anil Kumar Singh
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  3. Abha Shukla
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  4. Swaranjit Singh Cameotra
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Corresponding author

Correspondence to Swaranjit Singh Cameotra.

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Supplementary Fig. 1

Growth of C. albicans strains in the presence of biosurfactant produced by Bacillus amyloliquefaciens strain AR2. Growth was carried out at 30 °C with agitation rate of 200 rpm. Results are expressed as mean ± SD of values obtained from triplicate experiments. Supplementary material 1 (PPTX 109 kb)

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Rautela, R., Singh, A.K., Shukla, A. et al. Lipopeptides from Bacillus strain AR2 inhibits biofilm formation by Candida albicans . Antonie van Leeuwenhoek 105, 809–821 (2014). https://doi.org/10.1007/s10482-014-0135-2

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