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Micromonospora violae sp. nov., isolated from a root of Viola philippica Car

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Abstract

A novel actinomycete, designated strain NEAU-zh8T, was isolated from a root of Viola philippica Car collected in China and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-zh8T belongs to the genus Micromonospora, being most closely related to Micromonospora chokoriensis 2-9(6)T (99.9 %), Micromonospora saelicesensis Lupac 09T (99.3 %) and Micromonospora lupini Lupac 14NT (99.0 %). gyrB gene analysis also indicated that strain NEAU-zh8T should be assigned to the genus Micromonospora. The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-10(H4), MK-10(H2) and MK-10(H6). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were iso-C15:0, C16:0 and C17:0 10-methyl. A combination of DNA–DNA hybridization results and some physiological and biochemical properties indicated that strain NEAU-zh8T could be readily distinguished from the closest phylogenetic relatives. Therefore, it is proposed that strain NEAU-zh8T represents a novel Micromonospora species, for which the name Micromonospora violae sp. nov. is proposed. The type strain is NEAU-zh8T (=CGMCC 4.7102T=DSM 45888T).

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Acknowledgments

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Key Project for Basic Research (No. 2010CB126102), the National Key Technology R&D Program (No. 2012BAD19B06), the Program for New Century Excellent Talents in University (NCET-11-0953), the National Natural Science Foundation of China (No.31372006, 31171913 and 31071750), the Outstanding Youth Foundation of Heilongjiang Province (JC201201) and Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP).

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Yuejing Zhang, Hui Liu, Xinhui Zhang, Shurui Wang, Chongxi Liu, Xiangjing Wang & Wensheng Xiang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Chao Yu & Wensheng Xiang

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  1. Yuejing Zhang
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  2. Hui Liu
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  3. Xinhui Zhang
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Zhang, Y., Liu, H., Zhang, X. et al. Micromonospora violae sp. nov., isolated from a root of Viola philippica Car. Antonie van Leeuwenhoek 106, 219–225 (2014). https://doi.org/10.1007/s10482-014-0184-6

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  • DOI: https://doi.org/10.1007/s10482-014-0184-6

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