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Streptosporangium sonchi sp. nov. and Streptosporangium kronopolitis sp. nov., two novel actinobacteria isolated from a root of common sowthistle (Sonchus oleraceus L.) and a millipede (Kronopolites svenhedind Verhoeff)

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Abstract

Two novel actinobacteria, designated strains NEAU-QS7T and NEAU-ML10T, were isolated from a root of Sonchus oleraceus L. and a Kronopolites svenhedind Verhoeff specimen, respectively, collected from Wuchang, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The two strains were observed to form abundant aerial hyphae that differentiated into spherical spore vesicles. The phylogenetic analysis based on the 16S rRNA gene sequences of strains NEAU-QS7T and NEAU-ML10T showed that the two novel isolates exhibited 99.7 % 16S rRNA gene sequence similarity with each other and that they are most closely related to Streptosporangium shengliense NEAU-GH7T (99.1, 99.0 %) and Streptosporangium longisporum DSM 43180T (99.1, 99.0 %). However, the DNA–DNA hybridization value between strains NEAU-QS7T and NEAU-ML10T was 46.5 %, and the values between the two strains and their closest phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results, the two strains can be distinguished from each other and their closest phylogenetic relatives. Thus, strains NEAU-QS7T and NEAU-ML10T represent two novel species of the genus Streptosporangium, for which the names Streptosporangium sonchi sp. nov. and Streptosporangium kronopolitis sp. nov. are proposed. The type strains are NEAU-QS7T (=CGMCC 4.7142T =DSM 46717T) and NEAU-ML10T (=CGMCC 4.7153T =DSM 46720T), respectively.

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Acknowledgments

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Natural Science Foundation of China (No. 31471832, 31171913 and 31372006), the National Key Technology R&D Program (No. 2012BAD19B06), the Program for New Century Excellent Talents in University (NCET-11-0953), the Outstanding Youth Foundation of Heilongjiang Province (JC201201) and Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP), the Northeast Agricultural University Innovation Foundation For Postgraduate (yjscx14054), the Science and Technology Research Project of Heilongjiang Provincial Educational Commission (No. 12541001) and the Youth Science Foundation of Heilongjiang Province (No. QC2014C013). We are grateful to Prof. Aharon Oren for helpful advice on the specific epithet.

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    Authors and Affiliations

    1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

      Zhaoxu Ma, Hui Liu, Chongxi Liu, Junwei Zhao, Xin Wang, Jiansong Li, Xiangjing Wang & Wensheng Xiang

    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

      Hairong He & Wensheng Xiang

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    1. Zhaoxu Ma
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    Correspondence to Xiangjing Wang or Wensheng Xiang.

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    Zhaoxu Ma and Hui Liu have contributed equally to this work.

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    Ma, Z., Liu, H., Liu, C. et al. Streptosporangium sonchi sp. nov. and Streptosporangium kronopolitis sp. nov., two novel actinobacteria isolated from a root of common sowthistle (Sonchus oleraceus L.) and a millipede (Kronopolites svenhedind Verhoeff). Antonie van Leeuwenhoek 107, 1491–1499 (2015). https://doi.org/10.1007/s10482-015-0443-1

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