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Brachybacterium halotolerans sp. nov., a halotolerant, endophytic actinomycete isolated from branch of Bruguiera gymnoirhiza

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Abstract

A novel, aerobic, moderately halophilic Gram-positive actinomycete, strain MASK1Z-5T was isolated from a surface-sterilized branch of Bruguiera gymnoirhiza in Shankou Mangrove Nature Reserve, Guangxi, China. The taxonomic position of the strain was investigated using a polyphasic approach. Strain MASK1Z-5T tolerated up to 20% (w/v) NaCl (optimum 0–7%), and grew at pH 5.0–12.0 (optimum pH 7.0–8.0), 20–37 °C (optimum 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MASK1Z-5T belonged to the genus Brachybacterium, and showed the highest 16S rRNA gene sequence similarity of 98.0% to B. endophyticum M1HQ-2T. The cell-wall peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. MK-7 was the predominant menaquinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid and three unidentified lipids. The major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The DNA G + C content was calculated to be 71.8 mol% based on the whole genome sequence. The estimated values of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) based on whole genome sequences between strain MASK1Z-5T and B. endophyticum M1HQ-2T were 81.8% and 25.0%, respectively. The phenotypic, chemotaxonomic and genotypic properties clearly indicated that strain MASK1Z-5T represents a novel species within the genus Brachybacterium, for which the name Brachybacterium halotolerans sp. nov. is proposed. The type strain is MASK1Z-5T (= CGMCC1.18660T = JCM 34339T).

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Data availability statements

All data generated or analysed during this study are included in this published article, its supplementary information files and GenBank/EMBL/DDBJ.

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Funding

This research was supported by the National Natural Sciences Foundation of China (NSFC, Grant No. 81960642) and Guizhou Provincial Science and Technology Foundation (No. Qian Ke He Jichu [2019]1347).

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Authors and Affiliations

  1. Life Sciences Institute, Zunyi Medical University, Zunyi, 563006, People’s Republic of China

    Ming-Sheng Chen, Xiao-Hui Chen, Xiao-Rui Yan & Li Tuo

  2. Key Laboratory of Major Diseases in Children, Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), Beijing Key Laboratory of Pediatric Respiratory Infection Disease, National Clinical Research Center for Respiratory Diseases, National Center for Children’s Health, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing, 100045, People’s Republic of China

    Fei-Na Li

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Contributions

Ming-Sheng Chen, Xiao-Rui Yan and Li Tuo collected plant samples, isolated strains and preliminary identification. Ming-Sheng Chen performed experiments and wrote the manuscript. Li Tuo and Fei-Na Li performed genome and chemotaxonomic analyses. Ming-Sheng Chen, Xiao-Rui Yan and Xiao-Hui Chen, performed initial physiological and morphological analyses of the strain. Li Tuo conceived and designed the experiments and approved the final manuscript.

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Correspondence to Li Tuo.

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Chen, MS., Li, FN., Chen, XH. et al. Brachybacterium halotolerans sp. nov., a halotolerant, endophytic actinomycete isolated from branch of Bruguiera gymnoirhiza. Antonie van Leeuwenhoek 114, 875–884 (2021). https://doi.org/10.1007/s10482-021-01565-z

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