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Identification of potential plant extracts for anti-tick activity against acaricide resistant cattle ticks, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)

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Abstract

To develop an eco-friendly tick control method, seven plant extracts were prepared using 50 and 95 % ethanol and evaluated for acaricidal activity against cattle tick, Rhipicephalus (Boophilus) microplus. The adult immersion test was adopted for testing different extracts. Based on 72 h screening criterion, 95 % ethanolic extracts of Datura metel fruits and Argemone mexicana whole plant were found effective showing more than 50 % mortality of treated ticks. The 95 % ethanolic extracts of D. metel fruits and A. mexicana whole plant exhibited acaricidal and reproductive inhibitory effects on treated ticks. The LC90 values of D. metel and A. mexicana extracts were determined as 7.13 and 11.3 %, respectively. However, although both the extracts were found efficacious against deltamethrin-resistant IVRI-4 and multi-acaricide resistant IVRI-5 lines of R. (B.) microplus, they caused less mortality than treated ticks of the reference IVRI-I line. Phytochemical studies indicated the presence of alkaloids and glucosides in D. metel fruits and alkaloids, terpenoids, flavonoids and phenolics in A. mexicana whole plant extracts. The results indicated that these botanicals may play an important role in reducing the use of chemicals for tick control and possibly to manage resistant tick population in environment friendly manner.

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Acknowledgments

The authors are grateful to the Indian Council of Agricultural Research; New Delhi for funding through the World Bank funded National Agricultural Innovation Project No. [NAIP/Comp-4/C2066/2008–09] and National Agricultural Science Foundation Project No. [NFBSFARA/BSA-4004/2013–14].

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Entomology Laboratory, Division of Parasitology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243122, UP, India

    Srikanta Ghosh, Anil Kumar Sharma & Sachin Kumar

  2. Pharmacognosy and Ethnopharmacology Division, CSIR–National Botanical Research Institute, Lucknow, 226001, UP, India

    Shashi Shankar Tiwari, Bhanu Kumar, Sharad Srivastava & A. K. S. Rawat

  3. National Agricultural Science Foundation, Krishi Anusandhan Bhavan, New Delhi, 110012, India

    A. Bandyopadhyay

  4. Department of Veterinary Pharmacology, College of Veterinary and Animal Sciences, Pookode, Lakkidi, Wayanad, 673576, Kerala, India

    Sanis Julliet

  5. Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India

    Rajesh Kumar

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  1. Srikanta Ghosh
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Ghosh, S., Tiwari, S.S., Kumar, B. et al. Identification of potential plant extracts for anti-tick activity against acaricide resistant cattle ticks, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Exp Appl Acarol 66, 159–171 (2015). https://doi.org/10.1007/s10493-015-9890-7

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