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2,3,7,8-Tetrachlorodibenzo-p-dioxin regulates Bovine Herpesvirus type 1 induced apoptosis by modulating Bcl-2 family members

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Abstract

Exposure to environmental contaminants, like 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), leads to an increased susceptibility to infectious agents. Infection of bovine cells (MDBK) with Bovine Herpesvirus 1 (BHV-1) anticipates virus-induced apoptosis, suggesting an involvement of TCDD in virus infection. Herein we analyzed the effects of TCDD on apoptotic pathway in MDBK cells infected with BHV-1. After 12 h of infection, TCDD induced a significant increase in apoptotic cells. TCDD caused a dose-dependent up-regulation and anticipated activation of caspases 3, 8 and 9, with respect to unexposed groups. TCDD anticipated cleavage of PARP, compared to controls. Furthermore TCDD increased Bax and Bid levels, and decreased Bcl-2 and Bcl-XL levels. Such events took place earlier in exposed than unexposed cells. These results showed that TCDD influences BHV-1 induced apoptosis through members of Bcl-2 family and up-regulating activation of caspases.

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Abbreviations

TCDD:

2,3,7,8-Tetrachlorodibenzo-p-dioxin

BHV-1:

Bovine Herpesvirus 1

MDBK:

Madin-Darby bovine kidney cells

PARP:

Poly-(ADP-ribose) polymerase

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Acknowledgments

This work was supported by grants by Regione Campania for the project entitled: “Contaminanti organici persistenti nell’ambiente: Studio di coorte sullo stato sanitario e sui livelli di accumulo nel latte materno in gruppi di popolazione a differente rischio di esposizione nella Regione Campania”.

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Authors and Affiliations

  1. Section of Infectious Diseases, Department of Pathology and Animal Health, School of Veterinary Medicine, University of Naples “Federico II”, Via Delpino, 1, 80137, Naples, Italy

    Filomena Fiorito, Emma De Blasio, Giovanna Elvira Granato, Luisa De Martino, Serena Montagnaro & Ugo Pagnini

  2. Department of Experimental Medicine and Biochemical Sciences, University of Rome “Tor Vergata”, Via Montpellier 1, 00133, Rome, Italy

    Gabriella Marfè

  3. Department of Cellular and Molecular Pathology, IRCCS San Raffaele Pisana, Via dei Bonacolsi snc, 00163, Rome, Italy

    Marco Tafani

  4. Department of Structures, Function and Biological Technologies, School of Veterinary Medicine, University of Naples “Federico II”, Via Delpino, 1, 80137, Naples, Italy

    Salvatore Florio

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  1. Filomena Fiorito
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Correspondence to Filomena Fiorito.

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Fiorito, F., Marfè, G., De Blasio, E. et al. 2,3,7,8-Tetrachlorodibenzo-p-dioxin regulates Bovine Herpesvirus type 1 induced apoptosis by modulating Bcl-2 family members. Apoptosis 13, 1243–1252 (2008). https://doi.org/10.1007/s10495-008-0249-y

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