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Reprogramming of Human Retinal Pigment Epithelial Cells under the Effect of bFGF In Vitro

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We studied the effect of bFGF on human retinal pigment epithelial cells in vitro In ARPE-19 cells, enhanced expression of KLF4 mRNA and reduced expression of PAX6, MITF, and OTX2 mRNA specific for retinal pigment epithelium were observed after bFGF application. The expression of KLF4 mRNA peaked in 72 h after bFGF application and then sharply decreased, which was accompanied by a 3-fold increase in TUBB3 mRNA expression (neuronal marker). Immunocytochemical analysis showed that in the presence of bFGF, some cells retained epithelial properties and showed positive staining for connexin-43, while others had long axon-like processes and demonstrated positive staining for βIII-tubulin, which attests to their neuronal transdifferentiation. Despite the prevalence of the epithelial properties, ARPE-19 cells under the influence of bFGF can show proneuronal properties.

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Authors and Affiliations

  1. N. K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

    E. V. Shafei, A. M. Kurinov, A. V. Kuznetsova & M. A. Aleksandrova

Authors
  1. E. V. Shafei
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  2. A. M. Kurinov
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  3. A. V. Kuznetsova
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  4. M. A. Aleksandrova
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Corresponding author

Correspondence to M. A. Aleksandrova.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 2, pp. 128-136, June, 2017

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Shafei, E.V., Kurinov, A.M., Kuznetsova, A.V. et al. Reprogramming of Human Retinal Pigment Epithelial Cells under the Effect of bFGF In Vitro . Bull Exp Biol Med 163, 574–582 (2017). https://doi.org/10.1007/s10517-017-3852-5

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  • DOI: https://doi.org/10.1007/s10517-017-3852-5

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