Abstract
A more sensitive and convenient Coomassie Brilliant Blue (CBB) staining method for visualizing proteins was developed. Compared with the modifications include the supplement of 10% (v/v) methanol into the fixing solution, an increase of an additional sensitization step and CBB raised from 0.1 to 0.125%. The improved method can detect proteins at nanogram level. The improved method is more sensitive than Blue Silver and more convenient than the Silver protocol. Mass spectrometry results confirmed that it is suitable for subsequent proteomic research.
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Acknowledgments
This research was supported by the National High Technology and Research Development Program of China (“863” Project) and the Key Directional Research Project of CAS (Grant No. KSCX2-YW-N-003 and 013). We thank in particular Dr. Rongmin Zhao and Dr. Qiong Liu for critical reading and language polish of this article.
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Wang, X., Li, X. & Li, Y. A modified Coomassie Brilliant Blue staining method at nanogram sensitivity compatible with proteomic analysis. Biotechnol Lett 29, 1599–1603 (2007). https://doi.org/10.1007/s10529-007-9425-3
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DOI: https://doi.org/10.1007/s10529-007-9425-3