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Brewer’s spent grain as raw material for lactic acid production by Lactobacillus delbrueckii

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Abstract

Chemically pre-treated brewer’s spent grain was saccharified with cellulase producing a hydrolysate with approx. 50 g glucose l−1. This hydrolysate was used as a fermentation medium without any nutrient supplementation by Lactobacillus delbrueckii, which produced L-lactic acid (5.4 g l−1) at 0.73 g g−1 glucose consumed (73% efficiency). An inoculum of 1 g dry cells l−1 gave the best yield of the process, but the pH decrease affected the microorganism capacity to consume glucose and convert it into lactic acid.

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Acknowledgements

Thanks are given to CAPES, FAPESP, CNPq and NOVOZYMES.

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Authors and Affiliations

  1. Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, Brazil

    Solange I. Mussatto, Marcela Fernandes, Giuliano Dragone, Ismael M. Mancilha & Inês C. Roberto

  2. Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Vicosa, MG, Brazil

    Ismael M. Mancilha

Authors
  1. Solange I. Mussatto
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  2. Marcela Fernandes
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  3. Giuliano Dragone
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  4. Ismael M. Mancilha
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  5. Inês C. Roberto
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Correspondence to Solange I. Mussatto.

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Mussatto, S.I., Fernandes, M., Dragone, G. et al. Brewer’s spent grain as raw material for lactic acid production by Lactobacillus delbrueckii . Biotechnol Lett 29, 1973–1976 (2007). https://doi.org/10.1007/s10529-007-9494-3

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  • DOI: https://doi.org/10.1007/s10529-007-9494-3

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