Abstract
Hybrids between Escherichia coli and Lactobacillus brevis were generated via protoplast fusion. Growth kinetics of five hybrid strains and E. coli were used to evaluate the butanol tolerance of the novel strains under different conditions. The hybrid strains tolerated up to 2% (v/v) butanol compared to the 1% (v/v) maximum for E. coli. The growth inhibitory effects of butanol were also significantly less in several of the hybrids compared to E. coli. These results demonstrate the potential use of protoplast fusion to generate butanol-tolerant strains.
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Acknowledgements
We thank Dr. Albert Lee for assistance with experimentation and comments on the manuscript, Maria Priscilla Almario Falla for assistance with experimentation, and the National Science Foundation REU Grant 0552655 (MR) for funding.
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James Winkler and Matthew Rehmann contributed equally to this work.
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Winkler, J., Rehmann, M. & Kao, K.C. Novel Escherichia coli hybrids with enhanced butanol tolerance. Biotechnol Lett 32, 915–920 (2010). https://doi.org/10.1007/s10529-010-0247-3
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DOI: https://doi.org/10.1007/s10529-010-0247-3