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Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator

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Abstract

Whole whey hydrolyzed by Alcalase (WWH) was tested as a complex nitrogen source for the production of poly(3-hydroxybutyrate) (PHB) from waste frying oils by Cupriavidus necator H16. Addition of WWH (10 % (v/v) of cultivation media) supported the growth and PHB accumulation; PHB yields in Erlenmeyer flasks were more than 3.5-fold higher than in control cultivations. The positive influence of WWH on PHB production was confirmed in experiments performed in laboratory fermentor. C. necator cultivated with WWH produced 28.1 g PHB l−1 resulting in a very high product yield coefficient of 0.94 g PHB per g oil. Since PHB yields were ~40 % higher than in the control cultivation, WWH can be considered as an excellent inexpensive nitrogen source for PHB production by C. necator.

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Acknowledgments

This work was supported by project “Centre for Materials Research at FCH BUT” No. CZ.1.05/2.1.00/01.0012 from European Regional Development Fund (ERFD) and by the project “Excellent young researcher at BUT” No. CZ.1.07./2.3.00/30.0039. Authors thank to Dr. Libor Babak (Brno University of Technology, Czech Republic) for the provision of whole whey and to prof. Eva Strakova (University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic) for the analysis of amino acids composition.

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Authors and Affiliations

  1. Faculty of Chemistry, Materials Research Centre, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic

    Stanislav Obruca, Pavla Benesova & Ivana Marova

  2. Faculty of Chemistry, Institute of Chemistry and Technology of Environmental Protection, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic

    Jana Oborna

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  1. Stanislav Obruca
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  2. Pavla Benesova
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  3. Jana Oborna
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  4. Ivana Marova
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Correspondence to Stanislav Obruca.

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Obruca, S., Benesova, P., Oborna, J. et al. Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator . Biotechnol Lett 36, 775–781 (2014). https://doi.org/10.1007/s10529-013-1407-z

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  • DOI: https://doi.org/10.1007/s10529-013-1407-z

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