Abstract
A newly-isolated strain of Serratia marcescens, G12, was characterized for 2,3-butanediol (2,3-BD) production. In shake-flask and batch fermentations, 2,3-BD reached 48.5 and 51 g l−1, respectively. Low amounts of (~8 g l−1) of acetoin were also formed. In fed-batch fermentations, strain G12 produced 72.8 g 2,3-BD l−1 with glucose initially at 130 g l−1. When aeration rate was increased to 2.5 vvm for the fermentation process, 2,3-BD reached 87.8 g l−1 and the highest productivity was 1.6 g l−1 h−1. Acetoin was at 6.2 g l−1. G12 therefore may be a suitable candidate strain for large-scale production of 2,3-BD.
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This work was partly supported by The National Natural Science Foundation of China (Grant No. 31370205) and The National Key Technology R&D Program of China (Grant No. 2011Bacetoin11B05).
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Shi, L., Gao, S., Yu, Y. et al. Microbial production of 2,3-butanediol by a newly-isolated strain of Serratia marcescens . Biotechnol Lett 36, 969–973 (2014). https://doi.org/10.1007/s10529-013-1433-x
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DOI: https://doi.org/10.1007/s10529-013-1433-x