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Antioxidant properties of mesenchymal stem cells against oxidative stress in a murine model of colitis

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Abstract

Objective

To investigate the effects of oxidative stress injury in dextran sulfate sodium (DSS)-induced colitis in mice treated with mesenchymal stem cells (MSC).

Results

Mice exposed to oral administration of 2% DSS over 7 days presented a high disease activity index and an intense colonic inflammation. Systemic infusion of MSC protected from severe colitis, reducing weight loss and diarrhea while lowering the infiltration of inflammatory cells. Moreover, toxic colitis injury increased oxidative stress. Administration of DSS decreased reduced glutathione (GSH) and superoxide dismutase (SOD) activity, and increased thiobarbituric acid-reactive substances levels in the colon. No alteration was found in catalase (CAT) and glutathione peroxidase (GPx) activity. Otherwise, MSC transplantation was able to prevent the decrease of GSH levels and SOD activity suggestive of an antioxidant property of MSC.

Conclusion

The oxidative stress is a pathomechanism underlying the pathophysiology of colitis and MSC play an important role in preventing the impairment of antioxidants defenses in inflamed colon.

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Acknowledgements

This work was supported by Fundo de Incentivo à Pesquisa e Eventos (FIPE) of Hospital de Clínicas de Porto Alegre and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Authors and Affiliations

  1. Experimental Research Center, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos 2350, Porto Alegre, RS, CEP 90035-903, Brazil

    Fabiany da Costa Gonçalves, Natália Schneider Nunes, Fernanda Otesbelgue Pinto, Tuane Nerissa Alves Garcez & Ana Helena Paz

  2. Graduate Program in Gastroenterology and Hepatology Sciences, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2400, Porto Alegre, RS, CEP 90035-903, Brazil

    Fabiany da Costa Gonçalves, Natália Schneider Nunes & Ana Helena Paz

  3. Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2600, Porto Alegre, RS, CEP 90035-003, Brazil

    Mateus Grings & Guilhian Leipnitz

  4. Oral Pathology Department, School of Dentistry, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2492, Porto Alegre, RS, CEP 90035-007, Brazil

    Fernanda Visioli

Authors
  1. Fabiany da Costa Gonçalves
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  2. Mateus Grings
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  3. Natália Schneider Nunes
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  4. Fernanda Otesbelgue Pinto
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  5. Tuane Nerissa Alves Garcez
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  6. Fernanda Visioli
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  7. Guilhian Leipnitz
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  8. Ana Helena Paz
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Correspondence to Fabiany da Costa Gonçalves.

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da Costa Gonçalves, F., Grings, M., Nunes, N.S. et al. Antioxidant properties of mesenchymal stem cells against oxidative stress in a murine model of colitis. Biotechnol Lett 39, 613–622 (2017). https://doi.org/10.1007/s10529-016-2272-3

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  • DOI: https://doi.org/10.1007/s10529-016-2272-3

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