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Simultaneous nitrification and denitrification with different mixed nitrogen loads by a hypothermia aerobic bacterium

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Abstract

Microorganism with simultaneous nitrification and denitrification ability plays a significant role in nitrogen removal process, especially in the eutrophic waters with excessive nitrogen loads. The nitrogen removal capacity of microorganism may suffer from low temperature or nitrite nitrogen source. In this study, a hypothermia aerobic nitrite-denitrifying bacterium, Pseudomonas tolaasii strain Y-11, was selected to determine the simultaneous nitrification and denitrification ability with mixed nitrogen source at 15 °C. The sole nitrogen removal efficiencies of strain Y-11 in simulated wastewater were obtained. After 24 h of incubation at 15 °C, the ammonium nitrogen fell below the detection limit from an initial value of 10.99 mg/L. Approximately 88.0 ± 0.33% of nitrate nitrogen was removed with the initial concentration of 11.78 mg/L and the nitrite nitrogen was not detected with the initial concentration of 10.75 mg/L after 48 h of incubation at 15 °C. Additionally, the simultaneous nitrification and denitrification nitrogen removal ability of P. tolaasii strain Y-11 was evaluated using low concentration of mixed NH4+-N and NO3−–N/NO2−–N (about 5 mg/L-N each) and high concentration of mixed NH4+–N and NO3−–N/NO2−–N (about 100 mg/L-N each). There was no nitrite nitrogen accumulation at the time of evaluation. The results demonstrated that P. tolaasii strain Y-11 had higher simultaneous nitrification and denitrification capacity with low concentration of mixed inorganic nitrogen sources and may be applied in low temperature wastewater treatment.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 41371275) and the Scientific Research Innovation Project of Chongqing, China (No. CYB17063).

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Authors and Affiliations

  1. Chongqing Key Laboratory of Soil Multiscale Interfacial Process, College of Resources and Environment, Southwest University, NO 2 Tiansheng Road, Beibei District, Chongqing, 400716, China

    Tengxia He, Zhenlun Li, Deti Xie, Yi Xu, Qing Ye & Jiupai Ni

  2. Guizou Dejiang Secondary Vocational School, Guizhou, 565200, China

    Quan Sun

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  1. Tengxia He
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  2. Zhenlun Li
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  3. Deti Xie
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  4. Quan Sun
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  5. Yi Xu
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  6. Qing Ye
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  7. Jiupai Ni
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Correspondence to Zhenlun Li.

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He, T., Li, Z., Xie, D. et al. Simultaneous nitrification and denitrification with different mixed nitrogen loads by a hypothermia aerobic bacterium. Biodegradation 29, 159–170 (2018). https://doi.org/10.1007/s10532-018-9820-6

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  • DOI: https://doi.org/10.1007/s10532-018-9820-6

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