biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:629-632, 2005 | DOI: 10.1007/s10535-005-0064-z

In vitro regeneration and bulblet growth from lily bulbscale explants as affected by retardants, sucrose and irradiance

S. Kumar1,*, M. Kashyap1, D. R. Sharma1
1 Department of Biotechnology, University of Horticulture and Forestry, Solan, India

Bulbscales of oriental lily hybrid Star Gazer were used as the explants. Bulblets were formed on the basal portion of the excised bulbscales on MS medium supplemented with growth retardants, different sucrose concentrations and exposed to continuous light or dark. Alar, Cycocel and Paclobutrazol in concentration 1 mg dm-3 produced higher number of bulblets as compared to the control. The number of bulblets, however, decreased with the increase in concentration of the growth retardants. The number of bulblets was higher at 90 than at 60 g dm-3 sucrose and when the bulbscales were exposed to continuous light than to darkness. The growth retardants, higher sucrose concentration and continuous dark stimulated fresh mass of bulblets. The number of bulblets having roots and leaves decreased in medium with Alar, Cycocel and Paclobutrazol as compared to the control. A few bulblets produced roots and leaves in medium with 90 g dm-3 sucrose and none of the regenerated bulblets produced leaves under continuous dark.

Keywords: Lilium; Alar; Cycocel; Paclobutrazol; bulblet size
Subjects: bulb; growth retardants; in vitro culture, regeneration, organogenesis, morphogenesis; Lilium auratum × L. speciosum × L. rubellum hybrid Star Gazer; nutrient medium MS (Murashige and Skoog); photon flux density; retardant, bulblet growth; sucrose, bulblets

Received: June 1, 2004; Accepted: March 24, 2005; Published: December 1, 2005  Show citation

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Kumar, S., Kashyap, M., & Sharma, D.R. (2005). In vitro regeneration and bulblet growth from lily bulbscale explants as affected by retardants, sucrose and irradiance. Biologia plantarum, 49(4), 629-632. doi: 10.1007/s10535-005-0064-z
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