biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:93-97, 2005 | DOI: 10.1007/s10535-005-3097-4

The effect of NaCl on antioxidant enzyme activities in potato seedlings

H. Rahnama1,*, H. Ebrahimzadeh2
1 Agricultural Biotechnology Research Institute of Iran, Seed and Plant Improvement Institute Campus, Karaj, Iran
2 Department of Biology, Faculty of Science, University of Tehran, Tehran, Iran

The effect of NaCl on the growth and activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were investigated in the seedlings of four potato cultivars (Agria, Kennebec; relatively salt tolerant, Diamant and Ajax; relatively salt sensitive). The shoot fresh mass of Agria and Kennebec did not changed at 50 mM NaCl, whereas in Diamant and Ajax it decreased to 50 % of that in the controls. In Agria and Kennebec, SOD activity increased at 50 mM NaCl, but no significant changes observed in Diamant and Ajax. At higher NaCl concentration, SOD activity reduced in all cultivars. CAT and POD activities increased in all cultivars under salt stress. Unlike the other cultivars, in Ajax seedlings, APX activity increased in response to NaCl stress. We also observed new POD and SOD isoenzyme activities and changes in isoenzyme compositions under salt stress. These results suggest that salt-tolerant potato cultivars may have a better protection against reactive oxygen species (ROS) by increasing the activity of antioxidant enzymes (especially SOD) under salt stress.

Keywords: ascorbate peroxidase; catalase; peroxidase; salt stress; Solanum tuberosum; superoxide dismutase
Subjects: antioxidants, enzymes; ascorbate peroxidase; auxins; catalase; cultivar and genotype differences, salt stress; gibberellic acid, gibberellins; oxidative stress, antioxidants; peroxidase; potato, cultivars differing in sensitivity to salt stress; reactive oxygen species (ROS); salt stress, antioxidant enzymes; Solanum tuberosum; superoxide dismutase

Received: September 22, 2003; Accepted: April 27, 2004; Published: March 1, 2005  Show citation

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Rahnama, H., & Ebrahimzadeh, H. (2005). The effect of NaCl on antioxidant enzyme activities in potato seedlings. Biologia plantarum, 49(1), 93-97. doi: 10.1007/s10535-005-3097-4
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