biologia plantarum

Biologia plantarum 50:437-440, 2006 | DOI: 10.1007/s10535-006-0064-7

Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia

M. Faisal¹, M. Anis^1,*

¹ Plant Tissue Culture Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India

The effect of thidiazuron (TDZ) was studied on in vitro axillary shoot proliferation from nodal explant of Psoralea corylifolia - an endangered medicinal plant. Proliferation of shoots was achieved on Murashige and Skoog (MS) medium supplemented with 0.5, 1, 2, 3, 4 and 5 μM TDZ. The maximum number (13.6 ± 1.4) of shoots per explant were obtained from nodal segment cultured on 2 μM TDZ for 4 weeks and this increased to 29.7 ± 2.1 on hormone free MS medium after 8 weeks. The in vitro proliferated and elongated shoots were transferred individually on a root induction medium containing 0.5 μM indole-3-butyric acid (IBA) and within 4 weeks 4.5 ± 0.5 roots per shoot were produced. The regenerated plantlets were transferred to 1:1 soil and vermiculite mixture and acclimatized with 80 % survival rate. Fully acclimatized plants were grown in garden soil in greenhouse and their morphological and physiological parameters were comparable with seedlings.

Keywords: ex vitro transfer; indole-3-butyric acid; in vitro cultivation; plant regeneration

Subjects: auxins; ex vitro transfer; gas exchange; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog; (photosynthetic) photon flux density; Psoralea corylifolia; thidiazuron (TDZ)

Received: September 23, 2004; Accepted: March 30, 2005; Published: September 1, 2006  Show citation