biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:521-524, 2007 | DOI: 10.1007/s10535-007-0111-z

Direct plant regeneration from cucumber embryonal axis

A. Vasudevan1,*, N. Selvaraj3, A. Ganapathi2, C. W. Choi1, M. Manickavasagam2, S. Kasthurirengan2
1 Department of Biology and Medicinal Science, Pai Chai University, Daejeon, South Korea
2 Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
3 Department of Botany, Periyar E.V.R. College (Autonomous), Tiruchirappalli, Tamil Nadu, India

Embryonal axis explants from 2-d-old in vitro germinated seeds were used to induce multiple shoot production. The combination of 4.44 µM BA and 1.59 µM NAA in MS medium triggered the initiation of adventitious shoot buds. The explants with shoot buds produced maximum number of shoots (10.6 per explant) in MS medium supplemented with 4.44 µM BA and 0.065 mM L-glutamine in three successive transfers. The elongated shoots were rooted on MS medium with 4.92 µM IBA. Rooted plants were transferred to soil with a survival rate of 65 %.

Keywords: adventitious shoot bud; benzyladenine; Cucumis sativus L; L-glutamine; naphthalene acetic acid
Subjects: auxins; cucumber; Cucumis sativa; cytokinins; in vitro culture, regeneration, proliferation, differentiation

Received: June 1, 2005; Accepted: May 23, 2006; Published: September 1, 2007  Show citation

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Vasudevan, A., Selvaraj, N., Ganapathi, A., Choi, C.W., Manickavasagam, M., & Kasthurirengan, S. (2007). Direct plant regeneration from cucumber embryonal axis. Biologia plantarum, 51(3), 521-524. doi: 10.1007/s10535-007-0111-z
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    References

    1. Ananthakrishnan, G., Ravikumar, R., Vengadesan, G., Prem Anand, R., Ganapathi, A.: In vitro regeneration from embryo axis of cashewnut.-In: Kavi Kishor, R. (ed.): Plant Tissue Culture and Biotechnology. Pp. 123-128. University Press, Hyderabad 1999.
    2. Aziz, H.A., Mc Cown, B.H.: Hormonal response curves of shoot and callus cultures of cucumber (Cucumis sativus L.).-Sci. Hort. 20: 540-544, 1985.
    3. Buising, W., Shoemaker, R.C., Benbow, R.M.: Early events of multiple bud formation and shoot development in soybean embryonic axis treated with the cytokinin, 6-benzyl aminopurine.-Amer. J. Bot. 81: 1435-1444, 1994. Go to original source...
    4. Burza, W., Malepszy, S.: Direct plant regeneration from leaf explants in cucumber (Cucumis sativus L.) is free of stable genetic variation.-Plant Breed. 114: 341-345, 1995. Go to original source...
    5. Gambley, R.L., Dodd, W.A.: An In vitro technique for production of de novo of multiple shoots in cotyledon explants of cucumber (Cucumis sativus L.).-Plant Cell Tissue Organ Cult. 20: 177-183, 1990. Go to original source...
    6. Gamborg, O.L., Dav's, P., Stahelut, R.W.: Somatic embryogenesis in cell cultures of Glycine species.-Plant Cell Rep. 2: 209-212, 1968. Go to original source...
    7. Gomez, K.A., Gomez, K.A.: Statistical Procedures for Agricultural Research with Emphasis of Rice.-International Rice Research Institute, Los Baños 1975.
    8. Handley, L.W., Chambliss, O.L.: In vitro propagation of Cucumis sativus L.-Sci. Hort. 14: 22-23, 1979. Go to original source...
    9. Ignacimuthu, S., Franklin, G.: Regeneration of plantlets from cotyledon and embryonal axis explants of Vigna mungo L. Hepper.-Plant Cell Tissue Organ Cult. 55: 75-78, 1999. Go to original source...
    10. Kulothungan, S., Baskaran, A., Kasinathan, P., Shajahan, A., Ganapathi, A.: Morphogenetic studies on excised embryo culture of cowpea, Vigna unguiculata (L.) Walp.-Legume Res. 16(2): 71-74, 1993.
    11. Malepszy, S., Nadolska-Orezyk, A.: In vitro culture of Cucumis sativus VIII. Variation in the progeny of phenotypically not altered R1 Plants.-Plant Breed. 102: 66-72, 1989. Go to original source...
    12. Mathews, H.: Morphogenetic responses from in vitro cultured seedling explants of mungbean (Vigna radiata L.) Wilczek.-Plant Cell Tissue Organ Cult. 11: 238-246, 1987. Go to original source...
    13. Misra, A.K., Bhatnagar, S.P.: Direct shoot regeneration from leaf explant of cucumber (Cucumis sativus L.).-Phytomorphology 45: 47-55, 1995.
    14. Mohamed, F.M., Read, P.E., Coyne, P.D.: Plant Regeneration from in vitro culture of embryonic axis explants in common and tepary beans.-J. amer. Soc. hort. Sci. 177: 332-336, 1992. Go to original source...
    15. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    16. Plader, W., Burza, W., Rusinowski, Z.: The relationship between the regeneration system and genetic variability in cucumber (Cucumis sativus L.).-Euphytica 103: 9-15, 1998. Go to original source...
    17. Selvaraj, N.: In vitro culture and genetic transformation of cucumber (Cucumis sativus L.).-Ph.D. Thesis, Bharathidasan University, Tiruchirappalli 2002.
    18. Vasudevan, A., Selvaraj, N., Suresh Kumar, P., Ganapathi, A.: Multiple shoot formation from shoot tip explants of Cucumis sativus L.-Cucurbit Genet. Cooperat. Rep. 24: 8-12, 2001.
    19. Vasudevan, A., Selvaraj, N., Ganapathi, A., Kasthurirengan, S., Ramesh Anbazhagan, V., Manickavasagam, M.: Glutamine: a suitable nitrogen source for enhanced shoot multiplication in Cucumis sativus L.-Biol Plant. 48: 1215-128, 2004. Go to original source...
    20. Yang, Y.S., Wada, K., Futshuhara, Y.: Comparative studies of organogenesis and plant regeneration in various soybean explants.-Plant Sci. 72: 101-108, 1990. Go to original source...
    21. Ziv, M.: Micropropagation of Cucumis spp.-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry, High-Tech and Micropropagation III. Pp. 72-90. Springer-Verlag, Berlin-Heidelberg 1992. Go to original source...

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