biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 52:573-576, 2008 | DOI: 10.1007/s10535-008-0114-4

Response of the cherry rootstock to water stress induced in vitro

N. Sivritepe1,*, U. Erturk1, C. Yerlikaya2, I. Turkan3, M. Bor3, F. Ozdemir3
1 Department of Horticulture, Faculty of Agriculture, Uludag University, Bursa, Turkey
2 Chemical Engineering Department, Istanbul Technical University, Istanbul, Turkey
3 Department of Biology, Faculty of Science, Ege University, Izmir, Turkey

The in vitro response of sweet cherry (Prunus cerasus × P. canescens) rootstock Gisela 5 to increasing water deficit in the culture medium was studied. Water stress induced by the incorporation of 1, 2 and 4 % polyethylene glycol (PEG-8000) into the Murashige and Skoog medium was applied for 6 weeks. PEG-induced water stress reduced shoot dry mass, length, water content and relative chlorophyll content. Water stress also induced leaf necrosis without causing loss of viability in the explants. The increase in malondialdehyde content indicated oxidative stress. The activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POX) and glutathione reductase (GR) were also significantly elevated. The concentrations of K, Ca, Fe and Mn of shoots were decreased.

Keywords: antioxidant enzymes; chlorophyll; drought injury; growth; ion content; lipid peroxidation
Subjects: antioxidants, antioxidant enzymes; ascorbate peroxidase; calcium; catalase; chlorophyll a,b; glutathione reductase; lipid peroxidation; Prunus canescens; Prunus cerasus; rootstock; superoxide dismutase (SOD); sweet cherry; water stress

Received: April 18, 2007; Accepted: November 14, 2007; Published: September 1, 2008  Show citation

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Sivritepe, N., Erturk, U., Yerlikaya, C., Turkan, I., Bor, M., & Ozdemir, F. (2008). Response of the cherry rootstock to water stress induced in vitro. Biologia plantarum, 52(3), 573-576. doi: 10.1007/s10535-008-0114-4
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