biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:328-334, 2014 | DOI: 10.1007/s10535-014-0390-0

Effect of ultraviolet-B radiation on biomass production, lipid peroxidation, reactive oxygen species, and antioxidants in Withania somnifera

S. Takshak1, S. B. Agrawal1,*
1 Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi, India

The present study was aimed at understanding the effects of long term supplemental UV-B (3.6 kJ m-2 d-1) on biomass production, accumulation of reactive oxygen species, lipid peroxidation, and enzymatic antioxidants in leaves and roots of Withania somnifera (an indigenous medicinal plant). Under the UV-B treatment, a reduction in biomass and an increased malondialdehyde content (a characteristic of lipid peroxidation) were observed in both the shoots and roots. Amongst ROS, H2O2 content increased under UV-B in the leaves, whereas it decreased in the roots, and superoxide radical production rate decreased in both the plant parts. The activities of all enzymatic antioxidants tested (ascorbate peroxidase, catalase, glutathione reductase, peroxidase, polyphenol oxidase, and superoxide dismutase) increased under the UV-B treatment, the increase being greater in the roots.

Keywords: ascorbate peroxidase; catalase; glutathione reductase; malondialdehyde; oxidative stress; peroxidase; polyphenol oxidase; superoxide dismutase
Subjects: UV-B radiation; lipid peroxidation; reactive oxygen species; ascorbate peroxidase; catalase; glutathione reductase; malondialdehyde; peroxidase; polyphenol oxidase; superoxide dismutase; hydrogen peroxide; superoxide anion

Received: May 24, 2013; Revised: August 5, 2013; Accepted: September 30, 2013; Published: June 1, 2014  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Takshak, S., & Agrawal, S.B. (2014). Effect of ultraviolet-B radiation on biomass production, lipid peroxidation, reactive oxygen species, and antioxidants in Withania somnifera. Biologia plantarum, 58(2), 328-334. doi: 10.1007/s10535-014-0390-0
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abei, H.: Catalase in vitro. - Methods Enzymol. 105: 121-126, 1984. Go to original source...
    2. Agarwal, S.: Increased antioxidant activity in Cassia seedlings under UV-B radiation. - Biol. Plant. 51: 157-160, 2007. Go to original source...
    3. Agrawal, S.B., Rathore, D., Singh, A.: Effects of supplemental ultraviolet B and mineral nutrients on growth, biomass allocation and yield of wheat (Triticum aestivum L.). - Trop. Ecol. 45: 315-325, 2004.
    4. Agrawal, S.B., Rathore, D.: Changes in oxidative stress defence system in wheat (Triticum aestivum L.) and mung bean (Vigna radiata L.) cultivars grown with and without mineral nutrients and irradiated by supplemental ultraviolet-B. - Environ. exp. Bot. 59: 21-33, 2007. Go to original source...
    5. Agrawal, S.B., Singh, S., Agrawal, M.: Ultraviolet-B induced changes in gene expression and antioxidants in plants. - In: Jacquot, J.-P. (ed.): Advances in Botanical Research, Vol.52. Pp. 47-86. Academic Press, Burlington 2009. Go to original source...
    6. Alexieva, V., Sergiev, I., Mapelli, S., Karanov, E.: The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. - Plant Cell Environ. 24: 1337-1344, 2001. Go to original source...
    7. Anderson, M.E.: Glutathione. - In: Punchard, N.A., Kelly, F.J. (ed.): Free Radicals: a Practical Approach. Pp. 213-226. Oxford University Press, Oxford 1996.
    8. Asada, K.: Production and scavenging of active oxygen in chloroplasts. - In: Scandalios, J.G. (ed.): Molecular Biology of Free Radical Scavenging Systems. Pp. 173-192. Cold Spring Harbour Laboratory Press, New York 1992.
    9. Balakumar, T., Gayathri, B., Anbudurai, P.R.: Oxidative stress injury in tomato plants induced by supplemental UV-B radiation. - Biol. Plant. 39: 215-221, 1997. Go to original source...
    10. Ballaré, C.L., Caldwell, M.M., Flint, S.D., Robinson, S.A., Bornman, J.F.: Effects of solar ultraviolet radiation on terrestrial ecosystems. Patterns, mechanisms, and interactions with climate change. - Photochem. Photobiol. Sci. 10: 226-241, 2011. Go to original source...
    11. Britton, C., Mehley, A.C.: Assay of catalase and peroxidases. - In: Colowick, S.P., Kaplan, N.O., (ed.): Methods in Enzymology, Vol. II. Pp. 764-775. Academic Press, New York 1955. Go to original source...
    12. Cakirlar, H., Cícek, N., Ekmekcí, Y.: Is the induction of H2O2-detoxifyong antioxidant enzyme activities sufficient to protect barley cultivars from oxidative stress by UV-B irradiation alone or pre-treatment with high temperature and NaCl? - Turk. J. Biol. 35: 59-68, 2011. Go to original source...
    13. Casati, P., Lara, M.V., Andreo, C.S.: Regulation of enzymes involved in C4 photosynthesis and the antioxidant metabolism by UV-B radiation in Egeria densa, a submerged aquatic species. - Photosyn. Res. 71: 251-264, 2002. Go to original source...
    14. Elstner, E.F., Heupel, A.: Inhibition of nitrite formation from hydroxyl ammonium chloride: a simple assay for superoxide dismutase. - Anal. Biochem. 70: 616-620, 1976. Go to original source...
    15. Fridovich, I.: Superoxide dismutases. - Adv. Enzymol. 41: 35-97, 1974. Go to original source...
    16. Garg, N., Manchanda, G.: ROS generation in plants: boon or bane? - Plant Biosyst. 143: 81-96, 2009. Go to original source...
    17. Gaspar, T.H., Penel, C., Hagega, D., Greppin, H.: Peroxidases in plant growth, differentiation and development processes. - In: Lobarzewski, J., Greppin, H., Penel, C., Gaspar, T.H. (ed.): Biochemical, Molecular and Physiological Aspects of Plant Peroxidases. Pp. 249-280. Universitet de Genéve, Genéve 1991.
    18. Gill, S.S., Tuteja, N.: Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. - Plant Physiol. Biochem. 48: 909-930, 2010. Go to original source...
    19. Hagh, A.G., Khara, J., Darvishzadeh, R.: Effect of UV-B radiation on activity of antioxidant enzymes in four sunflower cultivars. - Int. J. agr. Res. 2: 528-534, 2012.
    20. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. Kinetics and stoichiometry of fatty acids peroxidation. - Arch Biochem. Biophys. 125: 189-198, 1968. Go to original source...
    21. Indrajith, A., Ravindran, K.C.: Antioxidant potential of Indian medicinal plant Phyllanthus amarus L. under supplementary UV-B radiation. - Recent Res. Sci. Technol. 1: 34-39, 2009.
    22. Jordan, B.R.: The effects of ultraviolet-B radiation on plants: a molecular perspective. - Adv. Bot. Res. 22: 97-162, 1996. Go to original source...
    23. Kataria, S., Jain, K., Guruprasad, K.N.: UV-B induced changes in antioxidant enzymes and their isoforms in cucumber (Cucumis sativus L.) cotyledons. - Indian J. Biochem. Biophys. 44: 31-37, 2007.
    24. Krasylenko, Y.A., Yemets, A.I., Blume, Y.B.: Cytoskeletonmediated signalling pathways in UV-B perception by plant cell. - Emir. J. Food Agr. 24: 557-564, 2012. Go to original source...
    25. Krupa, S.V. Kickert, R.N.: The greenhouse effect: impacts of ultraviolet-B (UV-B) radiation, carbon dioxide (CO2), and ozone (O3) on vegetation. - Environ. Pollut. 61: 263-393, 1989. Go to original source...
    26. Ktitorova, I.M., Skobeleva, O.V., Kanash, E.V., Bilova, T.E., Sharova, E.I.: Causes of root growth retardation induced by ultraviolet-B irradiation of shoots in barley seedlings. - Russ J. Plant Physiol. 53: 85-95, 2006. Go to original source...
    27. Kubi¶, J., Rybus-Zaj±c, M.: Drought and excess UV-B irradiation differentially alter the antioxidant system in cucumber leaves. - Acta biol. cracov. Ser. Bot. 50: 35-41, 2008.
    28. Kumar, K.B., Khan, P.A.: Peroxidase and polyphenol oxidase in excised ragi (Eleusine coracana cv. PR 202) leaves during senescence. - Indian J. exp. Bot. 20: 412-416, 1982.
    29. Kumari, R., Singh, S., Agrawal, S.B.: Combined effects of psoralens and ultraviolet-B on growth, pigmentation, and biochemical parameters of Abelmoschus esculentus L. - Ecotoxicol. Environ. Safety 72: 1129-1136, 2009a. Go to original source...
    30. Kumari, R., Singh, S., Agrawal, S.B.: Effects of supplemental ultraviolet B radiation on growth and physiology of Acorus calamus L. (sweet flag). - Acta biol. cracov. 51: 19-27, 2009b.
    31. Kumari, R., Singh, S., Agrawal, S.B.: Response of ultraviolet-B induced antioxidant defence system in a medicinal plant, Acorus calamus. - J. environ. Biol. 31: 907-911, 2010.
    32. Mackerness, A.H.S., Fred J.C., Jordan, B., Thomas, B.: Early signalling components in ultraviolet-B responses: distinct roles for reactive oxygen species and nitric oxide. - FEBS Lett. 489: 237-242, 2001. Go to original source...
    33. McKenzie, R.L., Aucamp, P.J., Bais, A.F., Björn, L.O., Ilyas, M., Madronich, S.: Ozone depletion and climate change: impacts on UV radiation. - Photochem. Photobiol. Sci. 10: 182-198, 2011. Go to original source...
    34. Mittler, R., Vanderauwera, S., Gollery, M., Van Breusegem, F.: Reactive oxygen gene network of plants. - Trends Plant Sci. 9: 490-498, 2004. Go to original source...
    35. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast. - Plant Cell Physiol. 22: 867-880, 1981.
    36. Nandi, P.K., Agrawal, M., Rao, D.N.: SO2 induced enzymatic changes and ascorbic acid oxidation in Oryza sativa. - Water Air Soil Pollut. 21: 25-32, 1984. Go to original source...
    37. Peiser, G.D., Yang, S.F.: Chlorophyll destruction in the presence of bisulphite and linoleic acid hydroperoxide. - Phytochemistry 17: 79-84, 1978. Go to original source...
    38. Rao, A.S.V., Reddy, A.R.: Glutathione reductase: a putative redox regulatory system in plant cells. - In: Khan, N.A., Singh, S., Umar, S. (ed.): Sulfur Assimilation and Abiotic Stresses in Plants. Pp. 111-147. Springer-Verlag, Berlin - Heidelberg 2008. Go to original source...
    39. Rao, M.V. Ormrod, D.P.: Impact of UV-B and O3 on the oxygen free radical scavenging system in Arabidopsis thaliana genotypes differing in flavonoids biosynthesis. - Photochem Photobiol. 62: 719-726, 1995. Go to original source...
    40. Ravindran, K.C., Indrajith, A., Balakrishnan, V., Venkatesan K., Kulandaivelu, G.: Determination of defense mechanism in Phaseolus trilobus seedlings treated under UV-B radiation. - Afr. Crop Sci. J. 16: 111-118, 2008. Go to original source...
    41. Ravindran, K.C., Indrajith, A., Pratheesh, P.V., Sanjiviraja, K., Balakrishnan, V.: Effect of ultraviolet-B radiation on biochemical and antioxidant defence system in Indigofera tinctoria L. seedlings. - Int. J. Engn. Sci. Technol. 2: 226-232, 2010. Go to original source...
    42. Rybus-Zaj±c, M.: Oxidative stress generation in Taxus baccata leaves affected by Pestalotiopsis funerea Desm. under different light conditions. - Dendrobiology 54: 51-56, 2005.
    43. Sharma, V., Sharma, S., Paliwal, R.: Withania somnifera: a rejuvenating ayurvedic medicinal herb for the treatment of various human ailments. - Int. J. Pharm. Tech. Res. 3: 187-192, 2011.
    44. Shim, I.S., Momose, Y., Yamamoto, A., Kim, D.W., Usui, K.: Inhibition of catalase activity by oxidative stress and its relationship to salicylic acid accumulation in plants. - Plant Growth Regul. 39: 285-289, 2003. Go to original source...
    45. Singh, A.: Growth, physiological and biochemical responses of three tropical legumes to enhanced UV-B radiation. - Can. J. Bot. 74: 135-139, 1996. Go to original source...
    46. Singh, R., Singh, S., Tripathi, R., Agrawal, S.B.: Supplemental UV-B radiation induced changes in growth, pigments and antioxidant pool of bean (Dolichos lablab) under field conditions. - J. environ. Biol. 32: 139-145, 2011.
    47. Stratmann, J.: Ultraviolet-B radiation co-opts defence signalling pathways. - Trends Plant Sci. 8: 526-533, 2003. Go to original source...
    48. Tang, K., Zhan, J.C., Yang, H.R., Huang, W.D.: Changes of resveratrol and antioxidant enzymes during UV-induced plant defense response in peanut seedlings. - J. Plant Physiol. 167: 95-102, 2010. Go to original source...
    49. Thypyapong, P., Hunt, M.D., Steffens, J.C.: Systemic wound induction of potato (Solanum tuberosum) polyphenol oxidase. - Phytochemistry 40: 673-676, 1995. Go to original source...
    50. Tripathi, R., Sarkar, A., Rai, S.P., Agrawal, S.B.: Supplemental ultraviolet B and ozone: impact on antioxidants, proteome and genome of linseed (Linum usitatissimum L. cv. Padmini). - Plant Biol. 13: 93-104, 2011. Go to original source...
    51. Xu, C., Natarajan, S., Sullivan, J.H.: Impact of solar ultraviolet-B radiation on the antioxidant defence system in soybean lines differing in flavonoids contents. - Environ. exp. Bot. 63: 39-48, 2008. Go to original source...
    52. Zhang, W.J., Björn, L.O.: The effect of ultraviolet radiation on the accumulation of medicinal compounds in plants. - Fitoterapia 80: 207-218, 2009. Go to original source...
    53. Zhao, Z.J., Song. Y.G., Liu, Y.L., Qiao, M., Zhai, X.L., Xiang, F.N.: The effect of elicitors on oleanolic acid accumulation and expression of triterpenoid synthesis genes in Gentiana straminea. - Biol. Plant. 57: 139-143, 2013. Go to original source...

    Return to the content