biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 60:788-792, 2016 | DOI: 10.1007/s10535-016-0605-7

Activity of DNA polymerase λ in spikelets of rice and maize

S. Bakshi1,*, S. Sihi2, D. N. Sengupta2
1 Department of Botany, Vidyasagar College for Women, Kolkata, India
2 Division of Plant Biology, Bose Institute, Kolkata, India

Involvement of DNA polymerase (pol) enzymes in meiotic DNA repair has not been clearly understood in plants. DNA polymerase λ is involved in short patch DNA synthesis and base excision repair in both plants and animals. The presence and activity of the pol λ enzyme was studied in a protein isolated from spikelets during flower development stages of rice (Oryza sativa L.) and maize (Zea mays L.). Western blot analysis showed a 2- to 2.5-fold higher accumulation of pol λ in spikelets than in shoots. Assays of pol and in-gel activity showed the dideoxynucleotide triphosphate sensitive pol λ enzyme in spikelets of both the plants. An enhanced presence of the enzyme and its high activity suggests an active role of pol λ in meiotic recombination during microspore development.

Keywords: DNA repair; in-gel activity assay; meiosis; Oryza sativa; Zea mays
Subjects: DNA polymerase λ; DNA repair; Western blot; maize; rice

Received: June 11, 2015; Revised: January 3, 2016; Accepted: January 18, 2016; Published: December 1, 2016  Show citation

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Bakshi, S., Sihi, S., & Sengupta, D.N. (2016). Activity of DNA polymerase λ in spikelets of rice and maize. Biologia plantarum, 60(4), 788-792. doi: 10.1007/s10535-016-0605-7
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