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Microfluidic Chip based direct triple antibody immunoassay for monitoring patient comparative response to leukemia treatment

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Abstract

We report a time and cost-efficient microfluidic chip for screening the leukemia cells having three specific antigens. In this method, the target blast cells are double sorted with immunomagnetic beads and captured by the 3rd antibody immobilized on the gold surface in a microfluidic chip. The captured blast cells in the chip were imaged using a bright-field optical microscope and images were analyzed to quantify the cells. First sorting was performed with nano size immunomagnetic beads and followed by 2nd sorting where micron size immunomagnetic beads were used. The low-cost microfluidic platform is made of PMMA and glass including micro size gold pads. The developed microfluidic platform was optimized with cultured B type lymphoblast cells and tested with the samples of leukemia patients. The 8 bone marrow samples of 4 leukemia patients on the initial diagnosis and on the 15th day after the start of the chemotherapy treatment were tested both with the developed microfluidic platform and the flow cytometry. A 99% statistical agreement between the two methods shows that the microfluidic chip is able to monitor the decrease in the number of blast cells due to the chemotherapy. The experiments with the patient samples demonstrate that the developed system can perform relative measurements and have a potential to monitor the patient response to the applied therapy and to enable personalized dose adjustment.

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Acknowledgements

Authors acknowledge TÜBİTAK (Project No: 115E020) for financial support. Authors also give thanks to Nazendenur Akşit and Ahsen Aydın for helping on taking the SEM images, Dr. Cengiz Gazeloğlu from Isparta Süleyman Demirel University for valuable discussions on statistical analysis.

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Authors and Affiliations

  1. BioMINDS (Bio Micro/Nano Devices and Sensors) Lab, Department of Electrical and Electronics Engineering, Abdullah Gül University, 38080, Kayseri, Turkey

    Kutay İçöz

  2. Bioengineering Department, Abdullah Gül University, 38080, Kayseri, Turkey

    Kutay İçöz & Ünal Akar

  3. Pediatric Hematology Department, Erciyes University, 38030, Kayseri, Turkey

    Ekrem Ünal

Authors
  1. Kutay İçöz
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  2. Ünal Akar
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  3. Ekrem Ünal
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Contributions

K.I designed the experiments, U. A, K. I and E. U conducted the experiments, interpreted the data and wrote the manuscript.

Corresponding author

Correspondence to Kutay İçöz.

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İçöz, K., Akar, Ü. & Ünal, E. Microfluidic Chip based direct triple antibody immunoassay for monitoring patient comparative response to leukemia treatment. Biomed Microdevices 22, 48 (2020). https://doi.org/10.1007/s10544-020-00503-6

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