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Histo-blood group antigen expression and proliferative activity of fibroblasts treated with dental monomers

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Abstract

The present work is focused on examining the effect of the structurally similar dental monomers bis-GA and bis-GMA on the expression of histo-blood group antigens (HBGAs) in comparison with fibroblast vitality and proliferation. The fibroblast cell line McCoy-Plovdiv was cultivated in a serum-free medium and was treated with both monomers. Cell vitality was measured by the crystal violet test. Mitotic index and cell morphology were assessed. An immunocytochemical technique was applied to follow the expression of proliferative antigens PCNA and Ki-67 and of HBGA. The expression level of HBGA was measured by an improved pixel selection algorithm with proprietary software. The lowest concentration of 2.5 μmol/L did not significantly affect morphology, vitality, or proliferation activity. Interestingly, the quantitative analysis revealed augmented expression of HBGA B at 2.5 μmol/L. The higher concentrations of the dental monomers reduced cell vitality and mitotic indices and altered proliferative antigen expression. Bis-GA proved to be more toxic than bis-GMA and caused more prominent alterations including greater enhancement of HBGA B expression. We present novel evidence for altered expression of proliferative antigens and enhanced expression of HBGA B in fibroblasts treated with dental monomers bis-GA and bis-GMA suggesting that these substances affect cell morphology, proliferative activity, and antigenic profile.

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Abbreviations

bis-GA:

2,2-bis[4-(2-hydroxy-3-acryloxypropoxy)phenyl]propane

bis-GMA:

2,2-bis[4-(2-hydroxy-3-methacryloxypropoxy)phenyl]propane

HBGA:

histo-blood group antigen(s)

PCNA:

proliferating cell nuclear antigen

References

  • Aronsson G, Dahlgren UI, Karlsson S. Human and rat mononuclear cell proliferation show different sensitivity, in vitro, to single constituents of dental composite resins. J Biomed Mater Res 2000;53:651–7.

    Article  PubMed  CAS  Google Scholar 

  • Docheva D, Draganov M, Murdjeva M. In vitro study on cytotoxic effect of some chemicals on serum McCoy and serum-free McCoy-Plovdiv cell culture systems. Folia Med (Plovdiv) 1999;41:43–5.

    CAS  Google Scholar 

  • Draganov M, Murdjeva M, Kamberov E. Development of a new serum-free cell culture system, McCoy-Plovdiv. In Vitro Cell Dev Biol Anim 2000;36:284–6.

    Article  PubMed  CAS  Google Scholar 

  • Draganov M, Murdjeva M, Sarafian V. Guidelines for cultivation and preservation of the serum-free cell line McCoy-Plovdiv. Cytotechnology 2003;42:163–7

    Article  Google Scholar 

  • Draganov M, Murdjeva M, Michailova-Topalska T. McCoy and McCoy-Plovdiv cell lines in experimental and diagnostic practice—past, present and perspectives. J Culture Collect 2005;4:3–16.

    Google Scholar 

  • Elias JM. Cell proliferation indexes: a biomarker in solid tumors. Biotech Histochem 1997;72:78–85.

    PubMed  CAS  Google Scholar 

  • Gerzina TM, Hume WR. Diffusion of monomers from bonding resin–resin composite combinations through dentine in vitro. J Dent 1996;24:125–8.

    Article  PubMed  CAS  Google Scholar 

  • Geurtsen W. Substances released from dental resin composites and glass ionomer cements. Eur J Oral Sci 1998;106:687–95.

    Article  PubMed  CAS  Google Scholar 

  • Hakomori SI. Tumor malignancy defined by aberrant glycosylation and sphingo(glyco)lipid metabolism. Cancer Res 1996;56:5309–18.

    PubMed  CAS  Google Scholar 

  • Hayrabedyan S, Kyurkchiev S, Kehayov I. Evaluation of IL-1A expression in endometriotic lesions using quantitative immunohistochemistry approach. Compt Rend Acad Bulg Sci 2006;59:229.

    CAS  Google Scholar 

  • Issa Y, Watts DC, Brunton PA, Waters CM, Duxbury AJ. Resin composite monomers alter MTT and LDH activity of human gingival fibroblasts in vitro. Dent Mater 2004;20:12–20.

    Article  PubMed  CAS  Google Scholar 

  • Jaffer F, Finer Y, Santerre JP. Interaction between resin monomers and commercial composite resins with human saliva derived esterases. Biomaterials 2002;23:1707–19.

    Article  PubMed  CAS  Google Scholar 

  • Kostoryz EL, Tong PY, Chappelow CC, Eick JD, Glaros AG, Yourtee DM. In vitro cytotoxicity of solid epoxy-based dental resins and their components. Dent Mater 1999;15:363–73.

    Article  PubMed  CAS  Google Scholar 

  • Lefebvre CA, Wataha JC, Bouillaguet S, Lockwood PE. Effects of long-term sub-lethal concentrations of dental monomers on THP-1 human monocytes. J Biomater Sci Polym Ed 1999;10:1265–74.

    PubMed  CAS  Google Scholar 

  • Mandel U, Petersen OW, Sorensen H, et al. Simple mucin-type carbohydrates in oral stratified squamous and salivary gland epithelia. J Invest Dermatol 1991;97:713–21.

    Article  PubMed  CAS  Google Scholar 

  • Marionneau S, Le Moullac-Vaidye B, Le Pendu J. Expression of histo-blood group A antigen increases resistance to apoptosis and facilitates escape from immune control of rat colon carcinoma cells. Glycobiology 2002;12:851–6.

    Article  PubMed  CAS  Google Scholar 

  • Matkowskyj KA, Schonfeld D, Benya RV. Quantitative immunohistochemistry by measuring cumulative signal strength using commercially available software Photoshop and Matlab. J Histochem Cytochem 2000;48:303–12.

    PubMed  CAS  Google Scholar 

  • Matkowskyj KA, Cox R, Jensen RT, Benya RV. Quantitative immunohistochemistry by measuring cumulative signal strength accurately measures receptor number. J Histochem Cytochem 2003;51:1041–8.

    PubMed  CAS  Google Scholar 

  • Mengwasser J, Sleeman JP. Expression of M-N#1, a histo-blood group B-like antigen, is strongly up-regulated in nonapoptosing mammary epithelial cells during rat mammary gland involution. Glycobiology 2001;11:441–9.

    Article  PubMed  CAS  Google Scholar 

  • Menoret A, Otry C, Labarriere N, et al. The expression of carbohydrate blood group antigens correlates with heat resistance. J Cell Sci 1995;108:1691–701.

    PubMed  CAS  Google Scholar 

  • Ortengen U, Welendorf H, Karlsson S, Ruyter IE. Water sorption and solubility of dental composites and identification of monomers released in an aqueous environment. J Oral Rehabil 2001;28:1106–15.

    Article  Google Scholar 

  • Pulgar R, Olea-Serano MF, Novillo-Fertell A, et al. Determination of bisphenol A and related aromatic compounds released from bis-GMA based composites and sealants by high performance liquid chromatography. Environ Health Perspect 2000;108:21–7.

    Article  PubMed  CAS  Google Scholar 

  • Reichl FX, Simon S, Esters M, et al. Cytotoxicity of dental composite (co)monomers and the amalgam component Hg(2+) in human gingival fibroblasts. Arch Toxicol 2006;80:465–72.

    Article  PubMed  CAS  Google Scholar 

  • Rose EC, Bumann J, Jonas IE, Kappert HF. Contribution to the biological assessment of orthodontic acrylic materials. Measurement of their residual monomer output and cytotoxicity. J Orofac Orthop 2000;61:246–57.

    Article  PubMed  CAS  Google Scholar 

  • Sarafian V, Marinova T. ABH histo-blood group antigens in human thymus involution. Arch Med Res 2006;37:844–7.

    Article  PubMed  CAS  Google Scholar 

  • Sarafian V, Dikov D, Karaivanov M, Belovejdov V, Stefanova P. Differential expression of ABH histo-blood group antigens and LAMPs in infantile hemangioma. J Mol Histol 2005;36:455–60.

    Article  PubMed  CAS  Google Scholar 

  • Schweikl H, Hiller KA, Bolay C, et al. Cytotoxic and mutagenic effects of dental composite materials. Biomaterials 2005a;26:1713–9.

    Article  CAS  Google Scholar 

  • Schweikl H, Altmannberger I, Hanser N, et al. The effect of triethylene glycol dimethacrylate on the cell cycle of mammalian cells. Biomaterials 2005b;26:4111–8.

    Article  CAS  Google Scholar 

  • Shintani H. HPLC analysis of toxic additives and residual monomers from dental plate. J Liquid Chromatogr 1995;18:613–26.

    Article  CAS  Google Scholar 

  • Sideridou I, Ahilias D. Elution study of unreacted bis-GMA, TEGDMA, UDMA, and bis-EMA from light cured dental resins and resin composites using HPLC. J Biomed Mater Res B 2005;74B:617–62.

    Article  CAS  Google Scholar 

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Acknowledgments

The study was supported by grant no. 22/2004 (Medical University – Plovdiv). The authors thank Dr. M. Draganov for providing the McCoy-Plovdiv cell line and for his important methodological assistance.

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Authors and Affiliations

  1. Department of Medical Biology, Medical University – Plovdiv, 15a V. Aprilov Blvd., Plovdiv, 4000, Bulgaria

    V. S. Sarafian, P. Ganchevska & M. Filipova

  2. Department of Chemistry and Biochemistry, Medical University – Plovdiv, Plovdiv, Bulgaria

    Y. Uzunova & L. Lukanov

  3. Laboratory of Molecular Immunology, Bulgarian Academy of Sciences, Sofia, Bulgaria

    S. Hayrabedyan

  4. Department of Endodontics and Operative Dentistry, Medical University – Plovdiv, Plovdiv, Bulgaria

    I. Filipov & S. Vladimirov

Authors
  1. V. S. Sarafian
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  2. Y. Uzunova
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  3. S. Hayrabedyan
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  4. P. Ganchevska
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  5. M. Filipova
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  6. I. Filipov
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  7. L. Lukanov
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  8. S. Vladimirov
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Correspondence to V. S. Sarafian.

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Sarafian, V.S., Uzunova, Y., Hayrabedyan, S. et al. Histo-blood group antigen expression and proliferative activity of fibroblasts treated with dental monomers. Cell Biol Toxicol 24, 27–37 (2008). https://doi.org/10.1007/s10565-007-9013-2

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  • DOI: https://doi.org/10.1007/s10565-007-9013-2

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