Abstract
Bacterial cellulose (BC) is a new biomaterial which has wide application potential in various industries. BC industrialization requires bacterial strains with high BC productivity. The objective of this study is to increase the BC yield of a Gluconoacetobacter xylinus strain through mutagenesis induced by high hydrostatic pressure (HHP) treatment. In this study, the parental strain in its exponential phase was treated at 250 MPa and 25 °C for 15 min to induce mutagenesis using a HHP machine. The HHP-treated strains were incubated in glucose agar plate at 30 °C for 4 days. After the incubation, 50 larger colonies in these plates were randomly selected and cultivated to produce BC membrane in a tailor-made glass vessel, and wet weights of the BC membranes were tested. Compared with the parental strain, 29 mutants showed higher BC yields, of which eight mutants with BC yield >130.00 g/L were initially screened and were then cultivated for five generations to test their genetic stabilities for BC production. Among the eight mutants, M438, a mutant which showed the highest average BC yield (158.56 g/L) and lowest coefficient of variation (2.4%) for five generations, was finally screened as objective mutant. HHP treatment can serve as an effective method to cause mutagenesis in BC-producing bacteria. The HHP-treated strains with significantly higher BC yield than parental strain can be screened from the HHP-induced mutants.
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References
Aloni Y, Cohen R, Benziman M, Delmer D (1983) Solubilization of the UDP-glucose: 1,4-β-d-glucan 4-β-d-glucoseyltransferase (cellulose synthase) from Acetobacter xylinum. J Biol Chem 258:4419–4423
Anicuta SG, Marta SF, Traian Z, Elena G (2007) Effect of electron beam irradiation on bacterial cellulose membranes used as transdermal drug delivery systems. Nucl Instrum Meth B 265:434–438
Benziman M, Rivertz B (1972) Factors affecting hexose phosphorylation in Acetobacter xylinum. J Bacteriol 111:325–333
Budhiono A, Rosidi B, Taher H, Iguchi M (1999) Kinetic aspects of bacterial cellulose formation in nata-de-coco culture system. Carbohydr Polym 40:37–143
Che LM, Ren FZ, Chen SW (2006) Breeding anti-postacidification strain from Lactobacillus bulgaricus by UV mutagenesis. Food Technol 27:109–112 (in Chinese)
De-Wulf P, Joris K, Vandamme EJ (1996) Improved cellulose formation by an Acetobacter xylinum mutant limited in (keto) gluconate synthesis. J Chem Technol Biotechnol 67:376–380
Gao X, Li J, Ruan KC (2001) Barotolerant E. coli induced by high hydrostatic pressure. Acta Biochem Biophys Sin 33:77–81 (in Chinese)
Gervilla R, Capellas M, Ferragut V, Guamis B (1997) Effect of high hydrostatic pressure on Listeria innocua 910 CECT inoculated into Ewe’s milk. J Food Protect 60:33–37
Gromet Z, Schramm M, Hestrin S (1957) Synthesis of cellulose by Acetobacter xylinum enzyme systems present in a crude extract of glucose-grown cells. Biochem J 67:679–689
Guo YX, Pan DD, Tanokura M (2009) Optimization of hydrolysis conditions for the production of the angiotensin-I converting enzyme (ACE) inhibitory peptides from whey protein using response surface methodology. Food Chem 114:328–333
Hayman MM, Anantheswaran RC, Knabel SJ (2007) The effects of growth temperature and growth phase on the inactivation of Listeria monocytogenes in whole milk subject to high pressure processing. Int J Food Microbiol 115:220–226
Hofherr LA, Glatz BA, Hammond EG (1983) Mutagenesis of strains of Propionibacterium to produce cold-sensitive mutants. J Dairy Sci 66:2482–2487
Hong F, Qiu KY (2008) An alternative carbon source from konjac powder for enhancing production of bacterial cellulose in static cultures by a model strain Acetobacter aceti subsp. xylinus ATCC 23770. Carbohydr Polym 72:545–549
Jonas R, Farah LF (1998) Production and application of microbial cellulose. Polym Degrad Stab 59:101–106
Keshk S, Sameshima K (2006) Influence of lignosufonate on crystal structure and productivity of bacterial cellulose in a static culture. Enzyme Microb Technol 40:4–8
Klemm D, Schumann D, Udhardt U, Marsch S (2001) Bacterial synthesized cellulose-artifical blood vessels for microsurgery. Prog Polym Sci 26:1561–1603
Lauro FM, Tran K, Vezzi A, Vitulo N, Valle G, Bartlett DH (2008) Large-scale transposon mutagenesis of Photobacterium profundum. SS9 reveals new genetic loci important for growth at low temperature and high pressure. J Bacteriol 190:1699–1709
Liu FZ, Zhang H, Mu KF (2008) Effects of high static pressure on beer yeast mutation breeding. Liquor-making Sci Tech 3:51–53 (in Chinese)
Miles AA, Misra SS, Irwin JQ (1938) The estimation of the bactericidal power of blood. J Hyg (London) 38:732–748
Nguyen VT, Gidley MJ, Dykes GA (2008) Potential of a nisin-containing bacterial cellulose film to inhibit Listeria monocytogenes on processed meats. Food Microbiol 25:471–478
O’Donovan GA, Kearney CL, Ingraham JL (1965) Mutants of Escherichia coli with high minimal temperatures of growth. J Bacteriol 90:611–616
Reyes DS, Celdrán AM, Pina-Pérez MC, Rodrigo D, López AM (2009) Modeling survival of high hydrostatic pressure treated stationary- and exponential-phase Listeria innocua cells. Innov Food Sci Emerg Technol 10:135–141
Ross P, Weinhouse H, Aloni Y, Michaeli D, Weinberger-Ohana P, Mayer R, Braun S, de Vroom E, van der Marel GA, van Boom JH, Benziman M (1987) Regulation of cellulose synthesis in Acetobacter xylinum by cyclic diguanylic acid. Nature 325:279–281
Valla S, Coucheron DH, Fjafrvik E, Kjosbakken J, Weinhouse H, Ross P, Amikam D, Benziman M (1989) Cloning of a gene involved in cellulose biosynthesis in Acetobacter xylinum: complementation of cellulose negative mutants by the UDPG pyrophosphorylase structural gene. Mol Genet Genetics 217:26–30
Vandamme EJ, Baets SD, Vanbaelen A, Joris K, De-Wulf P (1998) Improved production of bacterial cellulose and its application potential. Polym Degrad Stab 59:93–99
Vezzi A, Campanaro S, D’Angelo M, Simonato F, Vitulo N, Lauro FM, Cestaro A, Malacrida G, Simionati B, Cannata N, Romualdi C, Bartlett DH, Valle G (2005) Life at depth: photobacterium profundum genome sequence and expression analysis. Science 307:1459–1461
Wang SL, Wu XZ, Duan XC, Sun JS (2006) Mutagenic effects of ultra high pressure on laccase producing strains. Ind Microbiol 36:31–35 (in Chinese)
Wang L, Zhou LP, Chen Q (2008) Mutation breeding of monascus strains of high-yield of Monacolin K. Liquor-making Sci Technol 168:51–55 (in Chinese)
Wu RQ, Li ZX, Shao DY, Fan YL, Zhang XL, Li B, Bu LJ (2008a) Screening and primary identification of bacterial cellulose producing strain. China Brew 10:37–38 (in Chinese)
Wu RQ, Li ZX, Du SK, Xing XH, Shao DY, Fan YL, Li B, Zhang XL, Bu LJ (2008b) Optimization of bacterial cellulose fermentation medium and observation of bacterial cellulose ultra-micro-structure. Chin J Biotechnol 24:1068–1074 (in Chinese)
Yu XB, Bian YR, Quan WH, Liu W (1999) Breeding of high cellulose-producing strain Acetobacter xylinum. J Cellul Sci Technol 7:63–66 (in Chinese)
Acknowledgments
This research was financially supported by the Provincial Key Project for Science and Technology Development (grant number: 2005K03-G03), Shaanxi, China. The technical support of Dr. Xuchang Duan, associate professor of the College of Food Science and Engineering, Northwest A&F University, is also gratefully acknowledged.
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Wu, RQ., Li, ZX., Yang, JP. et al. Mutagenesis induced by high hydrostatic pressure treatment: a useful method to improve the bacterial cellulose yield of a Gluconoacetobacter xylinus strain. Cellulose 17, 399–405 (2010). https://doi.org/10.1007/s10570-009-9388-8
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DOI: https://doi.org/10.1007/s10570-009-9388-8