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Influence of Acute and Chronic Treadmill Exercise on Rat Plasma Lactate and Brain NPY, L-ENK, DYN A1–13

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SUMMARY

This study was designed to investigate the effect of acute and chronic high-intensity treadmill exercise on changes in plasma lactate and brain neuropeptide (NPY), leucine-enkephalin (L-ENK), and dynorpin A1–13 (DYN A1–13). Avidin–biotin complex (ABC) immunohistochemistry and image pattern analysis were used to observe the effect of chronic (total 7 weeks) and acute treadmill exercise (an initial speed of 15 m min−1 gradually increased to 35 m min−1 with 0°, 20–25 min per day duration) on the changes of NPY, L-ENK, and DYN A1–13 in different areas of rat brain. Plasma lactate was also measured in response to such exercise. Compared with preexercise control (P < 0.01), plasma lactate concentration significantly increased in the immediate postexercise; but it returned to the normal level soon after the 30 min postexercise. The content of NPY in paraventricular (PVN), dorsomedial (DMN), and ventromedial (VMN) hypothalamic nuclei continued to increase in 0, 30, and 180 min postexercise compared with preexercise control (P < 0.01). The content of L-ENK in caudate-putamen (CPu) significantly increased in the immediate postexercise compared with preexercise control (P < 0.01), but it gradually returned to the normal level after the 180 min postexercise. However, the content of DYN A1–13 in PVN rose substantially only in 30 min postexercise in comparison with the preexercise control (P < 0.01). Thus, different changes of NPY, L-ENK, and DYN A1–13 in response to such high-intensity exercise depend on the brain region and the time examined, especially, the contents of NPY in different brain regions continuously remain at a high level after such high-intensity exercise. And this high level might reduce energy expenditure and thus contribute to the stimulation of brain NPY neurons.

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ACKNOWLEDGMENTS

This project was supported by the National Natural Science Foundation of China (No. 30000216), Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutes of Ministry of Education, China, Foundation for the Authors of National Excellent Doctoral Dissertations of China (No. 200059), and Fok Ying Tong Education Foundation (No. 81037). Gratitude is expressed to Associate Professor Min-Juan Wang, working at Department of Educational Technology, San Diego State University, for her kindly revised the manuscript.

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  1. Department of TCM Diagnosis, Beijing University of Traditional Chinese Medicine, No. 11, Beisanhuan Donglu, Chaoyang District, Beijing, 100029, People's Republic of China

    Jia-Xu Chen, Xin Zhao, Guang-Xin Yue & Zhu-Feng Wang

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  1. Jia-Xu Chen
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  2. Xin Zhao
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  3. Guang-Xin Yue
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Correspondence to Jia-Xu Chen.

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Chen, JX., Zhao, X., Yue, GX. et al. Influence of Acute and Chronic Treadmill Exercise on Rat Plasma Lactate and Brain NPY, L-ENK, DYN A1–13 . Cell Mol Neurobiol 27, 1–10 (2007). https://doi.org/10.1007/s10571-006-9110-4

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  • DOI: https://doi.org/10.1007/s10571-006-9110-4

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