Abstract
Hypertension is a major health problem with increasing prevalence around the world. Tannic acid is water-soluble polyphenol that is present in tea, green tea, coffee, red wine, nuts, fruits and many plant foods. It has been reported to serve as an antioxidant or a pro-oxidant depending on the type of cells and its concentration. The purpose of our study was to evaluate the effect of tannic acid on systolic blood pressure, oxidative stress and some urinary parameters in the rat model of essential hypertension. Blood pressures of all rats were measured using the tail-cuff method. The nitric oxide synthase inhibitor N (omega)-nitro-L-arginine was administered orally at a dose of 0.5 g/l/day for 15 days to rats in order to create an animal model of hypertension. Tannic acid was intraperitoneally injected at a dose of 50 mg/kg for 15 days. Superoxide dismutase, catalase activity and the concentration of malondialdehyde (MDA) were determined in blood plasma and homogenates of heart, liver and kidney. In order to evaluate renal functions, urine pH, urine volume, urine creatine, uric acid, and urea nitrogen values were measured. Compared with the hypertension group, a decrease in MDA concentrations of heart tissue (p < 0.01), urea nitrogen values (p < 0.01) and urine volumes (p < 0.001) were established in hypertension + tannic acid group. There was also a decrease in blood pressure values (20th and 30th days) of this group, but there was no a statistical difference according to hypertension group. The findings of our research show the effect of tannic acid in lowering blood pressure in hypertensive rats.
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References
Baylis C, Mitruka B, Deng A (1992) Chronic blockade of nitric oxide synthesis in the rat produces systemic hypertension and glomerular damage. J Clin Invest 90:278–281. doi:10.1172/JCI115849
Cosan D, Soyocak A, Basaran A, Degirmenci I, Gunes HV (2009) The effects of resveratrol and tannic acid on apoptosis in colon adenocarcinoma cell line. Saudi Med J 30:191–195
Cosan DT, Bayram B, Soyocak A, Basaran A, Gunes HV, Degirmenci I, Musmul A (2010) Role of phenolic compounds in nitric oxide synthase activity in colon and breast adenocarcinoma. Cancer Biother Radiopharm 25:577–580. doi:10.1089/cbr.2010.0799
Cosan DT, Soyocak A, Basaran A, Degirmenci I, Gunes HV, Sahin FM (2011) Effects of various agents on DNA fragmentation and telomerase enzyme activities in adenocarcinoma cell lines. Mol Biol Rep 38:2463–2469. doi:10.1007/s11033-010-0382-x
Cowan MM (1999) Plant products as antimicrobial agents. Clin Microbiol Rev 12:564–582
Goth L (1991) A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 196:143–151
Gulcin I, Huyut Z, Elmastas M, Aboul-Enein HY (2010) Radical scavenging and antioxidant activity of tannic acid. Arab J Chem 3:43–53. doi:10.1016/j.arabjc.2009.12.008
Kashyap MK, Yadav V, Sherawat BS, Jain S, Kumari S, Khullar M, Sharma PC, Nath R (2005) Different antioxidants status, total antioxidant power and free radicals in essential hypertension. Mol Cell Biochem 277:89–99. doi:10.1007/s11010-005-5424-7
Kawakami K, Aketa S, Sakai H, Watanabe Y, Nishida H, Hirayama M (2011) Antihypertensive and vasorelaxant effects of water-soluble proanthocyanidins from persimmon leaf tea in spontaneously hypertensive rats. Biosci Biotechnol Biochem 75:1435–1439
Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J (2005) Global burden of hypertension: analysis of worldwide data. Lancet 365:217–223. doi:10.1016/S0140-6736(05)17741-1
Khan NS, Ahmad A, Hadi SM (2000) Anti-oxidant, pro-oxidant properties of tannic acid and its binding to DNA. Chem Biol Interact 125:177–189
Kiris I, Kapan S, Kilbas A, Yilmaz N, Altuntas I, Karahan N, Okutan H (2008) The protective effect of erythropoietin on renal injury induced by abdominal aortic-ischemia-reperfusion in rats. J Surg Res 149:206–213. doi:10.1016/j.jss.2007.12.752
Kour H, Perkins MJ (1991) The free radical chemistry of food additives. In: Arvoma OI, Halliwell B (eds) Free radicals and food additives. Taylor and Francis Ltd., London, pp 17–35
Krajka-Kuzniak V, Kaczmarek J, Baer-Dubowska W (2008) Effect of naturally occurring phenolic acids on the expression of glutathione S-transferase isozymes in the rat. Food Chem Toxicol 46:1097–1102. doi:10.1016/j.fct.2007.11.004
Labieniec M, Gabryelak T (2003) Effects of tannins on Chinese hamster cell line B14. Mutat Res 539:127–135. doi:10.1016/S1383-5718(03)00161-X
Labieniec M, Gabryelak T (2006) Oxidatively modified proteins and DNA in digestive gland cells of the fresh-water mussel Unio tumidus in the presence of tannic acid and its derivatives. Mutat Res 603:48–55. doi:10.1016/j.mrgentox.2005.10.013
Marienfeld C, Tadlock L, Yamagiwa Y, Patel T (2003) Inhibition of cholangiocarcinoma growth by tannic acid. Hepatology 37:1097–1104. doi:10.1053/jhep.2003.50192
Mihara M, Uchiyama M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:271–278
Oktar S, Ilhan S, Aksulu HE (2008) Clonidine prevents development of hypertension in N (omega)-nitro-L-arginine-treated rats. Anadolu Kardiyol Derg 8:104–110
Ozbayer C, Degirmenci I, Kurt H, Ozden H, Civi K, Basaran A, Gunes HV (2011) Antioxidant and free radical-scavenging properties of Stevia rebaudiana (Bertoni) extracts and L-NNA in streptozotocine–nicotinamide induced diabetic rat liver. Turk Klin Tip Bilim 31:51–60. doi:10.5336/medsci.2009-16216
Porteri E, Rizzoni D, De Ciuceis C, Boari GE, Platto C, Pilu A, Miclini M, Agabiti Rosei C, Bulgari G, Agabiti Rosei E (2010) Vasodilator effects of red wines in subcutaneous small resistance artery of patients with essential hypertension. Am J Hypertens 23:373–378. doi:10.1038/ajh.2009.280
Ribeiro MO, Antunes E, de Nucci G, Lovisolo SM, Zatz R (1992) Chronic inhibition of nitric oxide synthesis. A new model of arterial hypertension. Hypertension 20:298–303
Robinson HJ, Graessle OE (1943) Toxicity of tannic acid. J Pharmacol Exp Ther 77:63–69
Rodrigo R, Gil D, Miranda-Merchak A, Kalantzidis G (2012) Antihypertensive role of polyphenols. Adv Clin Chem 58:225–254
Ruiz-Gutierrez V, Vazquez CM, Santa-Maria C (2001) Liver lipid composition and antioxidant enzyme activities of spontaneously hypertensive rats after ingestion of dietary fats (fish, olive and high-oleic sunflower oils). Biosci Rep 21:271–285
Russo C, Olivieri O, Girelli D, Faccini G, Zenari ML, Lombardi S, Corrocher R (1998) Anti-oxidant status and lipid peroxidation in patients with essential hypertension. J Hypertens 16:1267–1271
Saravanakumar M, Raja B (2011) Veratric acid, a phenolic acid attenuates blood pressure and oxidative stress in L-NAME induced hypertensive rats. Eur J Pharmacol 671:87–94. doi:10.1016/j.ejphar.2011.08.052
Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide-dismutase. Clin Chem 34:497–500
Taffetani S, Ueno Y, Meng F, Venter J, Francis H, Glaser S, Alpini G, Patel T (2005) Tannic acid inhibits cholangiocyte proliferation after bile duct ligation via a cyclic adenosine 5′,3′-monophosphate-dependent pathway. Am J Pathol 166:1671–1679. doi:10.1016/S0002-9440(10)62477-7
Tanyer G (1985) Hematoloji ve laboratuvar. Ayyıldız matbaası A.Ş, Ankara
Touyz RM, Briones AM (2011) Reactive oxygen species and vascular biology: implications in human hypertension. Hypertens Res 34:5–14. doi:10.1038/hr.2010.201
World Health Organization (2003) The world health report 2002. Midwifery 19:72–73
Zatz R, Baylis C (1998) Chronic nitric oxide inhibition model six years on. Hypertension 32:958–964
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Turgut Coşan, D., Saydam, F., Özbayer, C. et al. Impact of tannic acid on blood pressure, oxidative stress and urinary parameters in L-NNA-induced hypertensive rats. Cytotechnology 67, 97–105 (2015). https://doi.org/10.1007/s10616-013-9661-4
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DOI: https://doi.org/10.1007/s10616-013-9661-4