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The brown seaweed Sargassum hemiphyllum exhibits α-amylase and α-glucosidase inhibitory activity and enhances insulin release in vitro

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Abstract

Diabetes is one of the most prevalent chronic diseases globally. In this study, major polyphenols (17.35 ± 0.93–36.66 ± 2.01 mg/g) and minor fucoxanthin (non detected 15.12 ± 0.09 mg/g) were isolated from water, ethanol, and acetone extracts (WES, EES, and AES, respectively) of Sargassum hemiphyllum. Inhibition of α-amylase, α-glucosidase, sucrose, and maltase activities and stimulation of insulin secretion was greater with AES than with WES or EES and correlated with polyphenol and fucoxanthin concentrations in extracts. Moreover, 250 μg/ml EES and AES significantly increased insulin secretion in the presence of 25 mg/ml glibenclamide to higher levels than those obtained with 50 mg/ml glibenclamide. None of the extracts exhibited cytotoxicity, exacerbated the side effects of glibenclamide, or inhibited glibenclamide-induced insulin secretion. These results suggested that the S. hemiphyllum extracts WES, EES, and AES could be used as pharmaceuticals and functional foods to reduce dosages of synthetic diabetes drugs.

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Authors and Affiliations

  1. Seafood Technology Division, Fisheries Research Institute, Council of Agriculture, Keelung City, Taiwan, ROC

    Pai-An Hwang, Yu-Lan Hung, Yi-Kuan Tsai & Shih-Yung Chien

  2. Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan, ROC

    Shih-Yung Chien

  3. Department of Food Science, National Taiwan Ocean University, No. 2, Beining Rd., Jhongjheng District, Keelung City, 202, Taiwan, ROC

    Zwe-Ling Kong

Authors
  1. Pai-An Hwang
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  2. Yu-Lan Hung
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  3. Yi-Kuan Tsai
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  4. Shih-Yung Chien
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  5. Zwe-Ling Kong
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Correspondence to Zwe-Ling Kong.

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Hwang, PA., Hung, YL., Tsai, YK. et al. The brown seaweed Sargassum hemiphyllum exhibits α-amylase and α-glucosidase inhibitory activity and enhances insulin release in vitro. Cytotechnology 67, 653–660 (2015). https://doi.org/10.1007/s10616-014-9745-9

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  • DOI: https://doi.org/10.1007/s10616-014-9745-9

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