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Induction of systemic resistance in banana (Musa spp.) against Banana bunchy top virus (BBTV) by combining chitin with root-colonizing Pseudomonas fluorescens strain CHA0

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Abstract

Pseudomonas fluorescens strains CHA0 and Pf1 were investigated for their biocontrol efficacy against Banana bunchy top virus (BBTV) in banana (Musa spp.) alone and in combination with chitin under glasshouse and field conditions. Bioformulation of P. fluorescens strain CHA0 with chitin was effective in reducing the banana bunchy top disease (BBTD) incidence in banana under glasshouse and field conditions. In addition to disease control, the bioformulation increased the economic yield significantly compared to the untreated control. Increased accumulation of oxidative enzymes, peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL), pathogenesis-related (PR) proteins, chitinase, β-1,3-glucanase and phenolics were observed in CHA0 bioformulation amended with chitin-treated plants challenged with BBTV under glasshouse conditions. Indirect ELISA indicated the reduction in viral antigen concentration in P. fluorescens strain CHA0 with chitin-treated banana plants corresponding to reduced disease ratings. The present study revealed that induction of defence enzymes by P. fluorescens with chitin amendment reduced the BBTD incidence and increased bunch yield in banana.

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Authors and Affiliations

  1. Department of Fruit Crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    Mathiyazhagan Kavino & Neelakandan Kumar

  2. Molecular Plant Virology lab, Department of Plant Pathology, National Chung Hsing University, Taichung, 11529, Taiwan

    Sankarasubramanian Harish

  3. AGROINNOVA, University of Turin, Turin, 10095, Italy

    Duraisamy Saravanakumar

  4. Director, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641 003, India

    Ramasamy Samiyappan

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  1. Mathiyazhagan Kavino
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  2. Sankarasubramanian Harish
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  3. Neelakandan Kumar
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  4. Duraisamy Saravanakumar
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  5. Ramasamy Samiyappan
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Correspondence to Mathiyazhagan Kavino.

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Kavino, M., Harish, S., Kumar, N. et al. Induction of systemic resistance in banana (Musa spp.) against Banana bunchy top virus (BBTV) by combining chitin with root-colonizing Pseudomonas fluorescens strain CHA0. Eur J Plant Pathol 120, 353–362 (2008). https://doi.org/10.1007/s10658-007-9223-8

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  • DOI: https://doi.org/10.1007/s10658-007-9223-8

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