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Genetic mapping of seedling and adult plant stem rust resistance in two European winter wheat cultivars

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Abstract

A recombinant inbred line (RIL) population derived from the cross Arina/Forno was field tested for 2 years against Puccinia graminis f. sp. tritici under artificially created epidemic conditions. Both parents showed intermediate adult plant stem rust responses and the RIL population showed continuous variation for this trait. Composite interval mapping identified genomic regions controlling low stem rust response on chromosomes 5B and 7D consistently across all experiments. These genomic regions were named QSr.Sun-5BL and QSr.Sun-7DS and explained on an average 12% and 26% of the phenotypic variation in adult plant stem rust response, respectively. QSr.Sun-5BL mapped close to Xglk0354 and was contributed by Arina. The Lr34-linked markers csLV34 and swm10 were closely associated with QSr.Sun-7DS suggesting the involvement of Lr34 in controlling adult plant stem rust response of cultivar Forno. Additional minor and inconsistent QTLs explaining variation in adult plant stem rust response were identified on chromosome arms 1AS and 7BL. The QTL located on chromosome 7BL corresponded to the stem rust resistance gene Sr17 carried by cultivar Forno. A seedling stem rust resistance gene carried by Arina, SrAn1, was ineffective under field conditions and was mapped on the long arm of chromosome 2A. Genotypes carrying combinations of QSr.Sun-5BL and QSr.Sun-7DS based on positive alleles of the respective closest marker loci Xglk0354 and XcsLV34 or Xswm10 exhibited a lower response than either parent indicating an additive effect of these genes. Transfer of these genes into cultivars carrying Sr2 would provide a more effective and durable resistance against the stem rust pathogen. Markers csLV34 and/or swm10 could be used in marker assisted selection of QSr.Sun-7DS in breeding programs.

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Acknowledgements

We thank the Australian Grains Research and Development Corporation for financial support through the Australian Winter Cereal Molecular Marker Program and the Australian Cereal Rust Control Program. We thank Dr. R. A. McIntosh for his critical comments. This work was also supported by a grant from the Swiss National Science Foundation 105620 to BK.

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Authors and Affiliations

  1. Faculty of Agriculture, Food and Natural Resources, Plant Breeding Institute-Cobbitty, University of Sydney, PMB11, Camden, NSW, 2570, Australia

    U. K. Bansal, H. Miah, R. F. Park & H. S. Bariana

  2. Institute of Plant Biology, University of Zürich, Zollikerstrasse 107, 8008, Zurich, Switzerland

    E. Bossolini & B. Keller

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  1. U. K. Bansal
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  2. E. Bossolini
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  3. H. Miah
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  4. B. Keller
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  5. R. F. Park
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  6. H. S. Bariana
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Correspondence to H. S. Bariana.

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Bansal, U.K., Bossolini, E., Miah, H. et al. Genetic mapping of seedling and adult plant stem rust resistance in two European winter wheat cultivars. Euphytica 164, 821–828 (2008). https://doi.org/10.1007/s10681-008-9736-z

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  • DOI: https://doi.org/10.1007/s10681-008-9736-z

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