Abstract
The present study examines the pattern of accumulation of cadmium (Cd) and its biochemical effects on selected tissues of a variety of African catfish (Clarias gariepinus), after exposure to various doses of Cd. The results obtained indicate that at the end of 21 days of exposure, the total tissue organ cadmium concentration followed the pattern kidney > gill > liver > muscle for each of the exposure concentrations. The levels of Cd in these organs were higher than those in ambient water. Moreover, while the rate of uptake of Cd increased with time in the kidney, liver and muscle, it decreased in the gill. Superoxide dismutase (SOD) was significantly elevated only in the kidney of catfish treated with 0.2 and 0.4 ppm of Cd for 7 days compared with the control. Conversely, gill SOD was significantly decreased in the same concentrations of Cd-treated catfish relative to the control. Statistically similar levels of SOD were observed in the liver, brain and muscle with all the treatments after the same duration of treatment. In the fish exposed for 21 days, SOD activity was significantly decreased in the kidney with a corresponding increase in lipid peroxidation (LPO), but it manifested only with the 0.2- and 0.4-ppm Cd treatment relative to the control. In the liver, however, Cd exposure significantly increased SOD in the 0.2- and 0.4-ppm treatments of the same duration. In conclusion, the present study indicates that the accumulation of Cd and its effect on SOD and LPO in C. gariepinus is dependent on concentration, tissue and time
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Abbreviations
- Cd:
-
Cadmium
- CAT:
-
Catalase
- LPO:
-
Lipid peroxidation
- MDA:
-
Malondialdehyde
- SOD:
-
Superoxide dismutase
References
Aksnes A, Njaa RL (1981) Catalase, glutathione peroxidase and superoxide dismutase in different fish species. Comp Biochem Physiol 69B:893–896
Asagba SO, Obi FO (2000) Effects of cadmium on kidney and liver cell membrane integrity and antioxidant enzyme status: implications for Warri River cadmium level. Trop J Environ Sci Health 3(1):33–39
Asagba SO, Obi FO (2001) Cadmium uptake: dose and time dependent tissue load and redistribution in the catfish, Clarias anguilaris (Line, 1758). Trop J Environ Sci Health 4(1):1–6
Asagba SO, Eriyamremu GE, Adaikpoh MA, Ezeoma A (2004) Levels of lipid peroxidation, superoxide dimutase and Na+/K+-ATPase in some tissues of rats exposed to a Nigerian-like diet and cadmium. J Biol Trace Elem Res 100:75–86
Atuma SO, Egborge ABM (1986) Insecticides and metals in Nigerian surface water-Warri River. Int J Environ Stud 27:139–142
Bagchi D, Bacghi M, Hassoun EA, Stohs SJ (1996) Cadmium induced excretion of urinary lipid metabolites, DNA damage, glutathione depletion and hepatic lipid peroxidation in Sprague–Dawley rats. Biol Trace Elem Res 52(2):143–154
Bartkowiak A, Bartkowiak J (1981) Superoxide dismutase and catalase activities in normal and cancerous tissues. Comp Biochem Physiol 70b:819–820
Crowe A, Morgan EH (1997) Effect of dietary cadmium on iron metabolism in geowing rats. Toxicol Appl Pharmacol 145(1):136–146
Edema CU, Egborge ABM (1992) Heavy metals concentration in water sediments and some shellfishes in Warri River and its tributaries. Proceedings of the National Seminar on Petroleum, Petroleum Training Institute, Effurun, Nigeria
Ezemonye LIN (1992) Heavy metals concentration in water, sediments and selected fish of Warri River and its tributaries. PhD thesis, University of Benin, Benin City, Nigeria
Fowler BA (1989) Biological roles of high affinity metal binding proteins in mediating cell injury. Comment Toxicol 3:47–68
Götz ME, Kunig G, Riederer GP, Youdim MBH (1994) Oxidative stress: free radical production in neural degeneration. Pharm Ther 63:37–122
Gupta S, Athar M, Behari JR, Srivastava RC (1991) Cadmium-mediated induction of cellular defense mechanism: a novel example for the development of adaptive response against a toxicant. Ind Health 29:1–9
Gutteridge JMC, Wilkins C (1982) Copper dependent hydroxy radical damage to ascorbic acid. Formation of a thiobarbituric acid reactive products. FEBS Lett 137:327–340
Halliwell B (1994) Free radicals and antioxidants. A personal view. Nutr Rev 52:253–265
Haspieler BM, Behar JV, Digiulio RT (1994) Glutathione dependent defense in channel catfish (Ictalurus punctatus) and brown bull head (Ameriurus nebulosus). Ecotoxicol Environ Saf 28:82–90
Jayakumar P, Paul VI (2006) Patterns of cadmium accumulation in selected tissues of the catfish Clarias batrachus (Linn.) exposed to sublethal concentration of cadmium chloride. Vet Archiv 76:167–177
Kaplan JH, Groves JN (1972) Liver and blood cell catalase activity of tumour bearing mice. Cancer Res 32:1190–1194
Klavercamp JE, McDonald WA, Duncan DA, Wagenann R (1984) Metallothionein and acclimation to heavy metals in fish: a review. In: Cairns VW, Hodson PV, Nriagu JO (eds) Contaminants effects on fisheries. Wiley, New York, pp 99–113
Misra HP, Fridovich I (1972) The role of superoxide ion in the auto-oxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 247:3170–3175
Pratap H, Bonga SE (1990) Effects of water-borne cadmium on plasma cortisol and glucose in the cichlid fish (Oreochromis mosambicus). Comp Biochem Physiol C 95:313–317
Price A, Lucas PW, Lea PJ (1990) Age dependent damage and glutathione metabolism in ozone fumigated barley: a leaf section approach. Exp Bot 41:1309–1317
Rao LM, Patnaik R (1999) Heavy metal accumulation in the catfish, Mystus vittatus (Bloch.) from Mehadrigedda Stream of Visakhapatinam, India. Pollut Res 19:325–329
Reinecke F, Levanets O, Olivier Y, Louw R, Semete B, Grobler A, Hidalg J, Olckers A, VanDer WF (2006) Metallothionein isoform 2A expression is inducible and protects against ROS-mediated cell death in retenone-treated Hela cells. J Biochem 395:405–415
Ryan PB, Huet N, Macintosh DL (2000) Longitudinal investigation of exposure to arsenic, cadmium and lead in drinking water. Environ Health Perspect 108:731–735
Shaik ZA, Vu TT, Zaman K (1999) Oxidative stress as a mechanism of chronic induced hepatotoxicity and renal toxicity and protection by antioxidants. Toxicol Appl Pharmacol 145:256–263
Sokal RR, Rohlf FU (1969) The principles and practice of statistics in biological research. Freeman, San Francisco
Timbrell JA (1991) Principles of biochemical toxicology, 2nd edn. Taylor & Francis, London
Tjalve H, Gottofrey J, Bjorklund I (1986) Tissue disposition of 109Cd2+ in the brown trout (Salmo trutta) studied by authoradiography and impulse counting. Toxicol Environ Chem 12:31–45
WHO (1992a) Environmental health criteria, 134, cadmium. World Health Organisation, Geneva
WHO (1992b) Environmental health criteria, 135, cadmium-environmental aspects. Cadmium-environmental aspects. World Health Organisation, Geneva
Wimmer U, Wang Y, Georgiev O, Schaffner W (2005) Two major branches of anti-cadmium defense in the mouse: MTF-1/metallothionein and glutathione. Nucleic Acids Res 33(18):5715–5727
Yamano T, Kosanke SD, Rikans LE (1999) Attenuation of cadmium induced liver injury in-senescent male fisher—344 rats. Role of metallothionein and glutathione. Toxicol Appl Pharmacol 161:225–230
Yin SJ, Chem CL, Sheu JY, Tseng WC, Lin TH (1999) Cadmium induced renal lipid peroxidation in rats and protection by selenium. J Toxicol Environ Health 57:403–413
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Asagba, S.O., Eriyamremu, G.E. & Igberaese, M.E. Bioaccumulation of cadmium and its biochemical effect on selected tissues of the catfish (Clarias gariepinus). Fish Physiol Biochem 34, 61–69 (2008). https://doi.org/10.1007/s10695-007-9147-4
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DOI: https://doi.org/10.1007/s10695-007-9147-4