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Dietary lysine requirement of fingerling Catla catla (Hamilton) based on growth, protein deposition, lysine retention efficiency, RNA/DNA ratio and carcass composition

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Abstract

A 12-week experiment was conducted to quantify dietary lysine requirement of fingerling Catla catla (3.65 ± 0.05 cm; 0.58 ± 0.02 g) by feeding casein–gelatine-based diets (33.0 % crude protein; 14.3 kJ/g digestible energy) with six levels of l-lysine (1.25, 1.50, 1.75, 2.00, 2.25 and 2.50 % dry diet). The experiment was conducted in eighteen 70-L indoor polyvinyl circular troughs provided with a water flow-through system (1–1.5 L/min). Live weight gain (LWG), feed conversion ratio (FCR), protein deposition (PD), lysine retention efficiency (LRE%) and RNA/DNA ratio were used as the response criteria. Second-degree polynomial regression analysis at 95 % maximum and minimum response of LWG and FCR data exhibited the lysine requirement between 1.8 and 1.9 % dry diet, corresponding to 5.5–5.7 % dietary protein. Regression analysis of PD, LRE and RNA/DNA ratio yielded the requirement between 1.7 and 1.8 % dry diet, corresponding to 5.2–5.5 % dietary protein. Since live weight gain and protein deposition are the key parameters for estimating nutrient requirement, these tools were used to recommend the lysine requirement of fingerling C. catla which ranges between 1.7 and 1.8 % dry diet. Data generated during this study will be useful to formulate lysine-balanced feed for intensive culture of this fish.

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Abbreviations

LWG:

Live weight gain

FCR:

Feed conversion ratio

PD:

Protein deposition

LRE:

Lysine retention efficiency

MS-222:

Tricaine methanesulphonate

VSI:

Viscerosomatic index

HSI:

Hepatosomatic index

CF:

Condition factor

CP:

Crude protein

TCA:

Trichloroacetic acid

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Acknowledgments

The authors are grateful to the Chairman, Department of Zoology, Aligarh Muslim University, Aligarh, India for providing necessary laboratory facilities and also to Prof. John E. Halver for supporting the Fish Nutrition Research Programme at this laboratory. We also gratefully acknowledge the financial assistance from Maulana Azad National Fellowship-University Grant Commissions for minority students for the year 2010–2011 awarded to one of us (Seemab Zehra) and generous funding received under DST-FIST Programme, New Delhi in the priority area of Fish Nutrition.

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  1. Fish Nutrition Research Laboratory, Department of Zoology, Aligarh Muslim University, Aligarh, 202 002, India

    Seemab Zehra & Mukhtar A. Khan

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  1. Seemab Zehra
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  2. Mukhtar A. Khan
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Correspondence to Mukhtar A. Khan.

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Zehra, S., Khan, M.A. Dietary lysine requirement of fingerling Catla catla (Hamilton) based on growth, protein deposition, lysine retention efficiency, RNA/DNA ratio and carcass composition. Fish Physiol Biochem 39, 503–512 (2013). https://doi.org/10.1007/s10695-012-9715-0

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