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Expression of mep50 in adult and embryos of medaka fish (Oryzias latipes)

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Abstract

Protein arginine methylation is important for gene regulation and biological processes. Methylosome protein 50 (Mep50) is identified as a partner of protein arginine methyltransferase 5 (Prmt5), a major enzyme capable of symmetric dimethylation, in mammals and Xenopus. The isolation and characterization of medaka mep50 were reported in this paper. Medaka Mep50 is a homolog of human MEP50 with six WD40 domains. Medaka mep50 was ubiquitously expressed in the adult tissues and had maternal origin with continuous and dynamical expression during embryonic development detected by RT-PCR and in situ hybridization. A strong interaction of medaka Mep50 and Prmt5 was shown by yeast two hybridization. The expression pattern of mep50 is similar to that of prmt5 in medaka. The results suggested that medaka Mep50 could be a partner of Prmt5 and might play major roles in a variety of tissues in medaka.

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Abbreviations

AA:

Amino acids

BCIP:

5-Bromo-4-chloro-3-indolyl phosphate

bp:

Base pairs

DIG:

Digoxigenin

FCP:

TFIIF-associating carboxyl-terminal domain phosphatase

hpf:

Hours post-fertilization

Mep50:

Methylosome protein 50

NBT:

Nitroblue tetrazolium

ORF:

Open reading frame

PBS:

Phosphate-buffered saline

PCR:

Polymerase chain reaction

PGC:

Primordial germ cell

Prmt:

Protein arginine methyltransferase

RT-PCR:

Reverse transcription polymerase chain reaction

snRNP:

Small nuclear ribonucleic protein

SSC:

A buffer containing sodium citrate and sodium chloride

WD40:

A domain with approximately 40 AAs and usually ending with a tryptophan (W) and aspartic acid (D)

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Acknowledgments

This work was supported by the Natural Science Foundation of China (Grant No. 31272645).

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    Authors and Affiliations

    1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China

      Nana Cheng, Maomao Guo, Pei Chang, Xueyan Zhang, Runshuai Zhang, Chao Qi, Xueping Zhong, Qingchun Zhou & Haobin Zhao

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    1. Nana Cheng
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    Correspondence to Haobin Zhao.

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    Nana Cheng and Maomao Guo have contributed equally to this article.

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    Cheng, N., Guo, M., Chang, P. et al. Expression of mep50 in adult and embryos of medaka fish (Oryzias latipes). Fish Physiol Biochem 42, 1053–1061 (2016). https://doi.org/10.1007/s10695-016-0196-4

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    • DOI: https://doi.org/10.1007/s10695-016-0196-4

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