Abstract
Polyploidisation is assumed to have played a significant role in the evolution of hybrid asexual lineages. The virtual absence of natural asexual systems in which more than a single ploidy level successfully establishes successful independent clonal lineages is generally explained by the strong effects of polyploidisation on fitness. Experimental crosses were made between diploid and triploid asexual Cobitis elongatoides × C. taenia hybrids (female) and both parental spined loach species (male). Genotyping of the progeny using allozymes and multilocus DNA fingerprinting, along with flow cytometric measurement of ploidy level, demonstrated the occurrence of gynogenetic reproduction in both female biotypes. The incorporation of the sperm genome occurred in some progeny, giving rise to a higher ploidy level, but the rate of polyploidisation differed significantly between the diploid and triploid females. These outcomes are consistent with the existence of developmental constraints on tetraploidy, which determine the rarity of tetraploids in natural populations. No cases of ploidy level reduction were observed. Since diploid and triploid hybrid populations occur where the lack of potential progenitor excludes the possibility of de novo origin, it is probable that both diploid and triploid females can establish successful clonal lineages. Spined loaches represent a unique example, among asexual vertebrates, where more than one ploidy level can establish persistent clonal lineages, which are reproductively independent of one another.
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Acknowledgements
We are deeply obliged to Jana Pivoňková and Vlastimil Šlechta for laboratory assistance, and to M. Stöck for his critical reading of the manuscript and for his useful comments. Three anonymous referees provided a useful criticism on the previous version of the manuscript. The research was supported by the Ministry of Education, Youth and Sport of the Czech Republic, MSM 126100001, Grant Agency of the Czech Republic No. GAČR 206/05/P586, and by the German Research Foundation in the SFB 567 ‘Mechanisms of interspecific interactions’ and the EU Marie Curie Research and Training Network ‘SexAsex’ (MCRTN-CT-2004-512492). The study was supported in part by USB RIFCH project No. MSM6007665809 (to M.F.). The Laboratory of Fish Genetics at Liběchov receives continuous support from the Academy of Sciences of the Czech Republic (IRP lIAPG No. AV0Z50450515). We also thank www.smartenglish.co.uk for linguistic advice in the preparation of this manuscript.
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Janko, K., Bohlen, J., Lamatsch, D. et al. The gynogenetic reproduction of diploid and triploid hybrid spined loaches (Cobitis: Teleostei), and their ability to establish successful clonal lineages—on the evolution of polyploidy in asexual vertebrates. Genetica 131, 185–194 (2007). https://doi.org/10.1007/s10709-006-9130-5
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DOI: https://doi.org/10.1007/s10709-006-9130-5